山麦胶囊对肾性高血压大鼠肾内小动脉血管重构的影响

Effect of Shanmai Capsules on the Renal Arteriole Remodeling in Renovascular Hypertensive Rats

目的:探讨山麦胶囊对肾性高血压大鼠肾内小动脉血管重构的影响。方法:建立"两肾一夹"肾性高血压大鼠模型,随机分为模型对照组8只,卡托普利组(0.007 g·kg-1)8只,山麦胶囊高剂量组(1.8 g·kg-1)8只,中剂量组(0.9 g·kg-1)7只,低剂量组(0.45 g·kg-1)6只;另设假手术组10只。于造模第5周开始每日灌胃给药,定期测定大鼠尾动脉收缩压(SBP),给药4周后处死大鼠,酶联免疫法测定血浆中血管紧张素Ⅱ(AngⅡ)和内皮素(ET)含量,组织切片图像分析法评价肾内小动脉重构指标及其形态改变。结果:经肾动脉缩窄术后大鼠血压显著增高(P<0.01)。与模型对照组比较,山麦胶囊呈可逆性及浓度依赖性地降低肾性高血压大鼠的血压、血浆中AngⅡ和ET含量及肾内小动脉中层厚度(Mt),同时增加血管腔径(RAD)和腔面积(Lcsa),差异有统计学意义(P<0.01,P<0.05)。结论:山麦胶囊可以有效预防肾性高血压大鼠肾内小动脉血管重构,该作用可能与其降压作用及拮抗体内AngⅡ和ET水平有关。

Objective： To observe the effect of Shanmai capsules on the renal arteriole remodeling in renovascular hypertensive rats.Method： The two-kidney one-clip（2K1C） renovascular hypertensive rats were randomly divided into model group（n = 8）,captopril group（0.007 g·kg-1,n = 8）,Shanmai capsules high（1.8 g·kg-1,n =8）,middle（0.9 g·kg-1,n = 7）,low（0.45 g·kg-1,n = 6） dosage group and the sham control group（n = 10）.Treatment was started at the 5th week after surgery by intragastric administration.Systolic blood pressure（SBP） was measured periodically.Rats were sacrificed after the last administration.AngiotensinⅡ（Ang Ⅱ） and endothelin（ET） were measured by enzyme-linked immunosorbent assays（ELISA）.The morphometric measurements were performed in the renal arteriole remodeling.Result： The blood pressure of rats after renal artery narrow operation was significantly higher than before（P 0.01）.Compared with model group,the antihypertensive effect of Shanmai capsules on renal hypertensive rats was reversible with a concentration dependent manner.The Ang Ⅱ and ET level in plasma and the renal arterial media thickness（Mt） were decreased,and lumen diameter（RAD） and luminal cross-sectional area（Lcsa） were increased,differences were statistically significant（P 0.01,P 0.05）.Conclusion： Shanmai capsules could inhibit the renal arteriole remodeling in renovascular hypertensive rats,and its mechanism may be related to the antihypertensive effect and reducing the levels of AngⅡand ET in vivo.