铁皮石斛对ApoE^(-/-)小鼠血脂及TNF-α,IL-6的影响

Effects of Dendrobii Officinalis Caulis on Serum Lipid,TNF-α and IL-6 in Apolipoprotein E-Deficient Mice

目的:考察铁皮石斛对ApoE-/-小鼠血脂、炎症相关蛋白的影响,探讨其抗动脉粥样硬化的作用机制。方法:将ApoE-/-小鼠以高脂饲喂养4 w构建AS小鼠模型,分别用辛伐他汀(3 mg·kg-1)、铁皮石斛(1.5,3 g·kg-1)对小鼠灌胃给药,8w后处死,酶法检测血清血脂;ELISA检测血清中TNF-α,IL-6表达;苏丹IV染色观察主动脉瓣处斑块形成情况;HE和油红O染色观察小鼠主动脉管腔内粥样斑块;免疫组化法检测TNF-α和IL-6在主动脉壁的表达。结果:铁皮石斛可显著降低小鼠血清中TG,TCHOL,LDL-C水平,下调血清中TNF-α,IL-6表达;可明显减少ApoE-/-小鼠主动瓣处粥样斑块的面积和ApoE-/-小鼠主动脉管腔内粥样斑块的面积,可明显降低TNF-α,IL-6在主动脉内表达。结论:铁皮石斛能有效降低ApoE-/-小鼠血清中有关脂质含量,降低血清和主动脉内TNF-α,IL-6的表达,降低炎症反应的发生,缓解动脉粥样硬化损伤。

Objective： To investigate effects of Dendrobii Officinalis Caulis on serum lipid and inflammation-related proteins in ApoE-/- mice,and explore its anti-atherosclerotic mechanisms.Method： ApoE-/- mice were fed a high-cholesterol diet for 4 weeks to induce atherosclerosis mode,then they were given simvastatin（3 mg·kg-1）,Dendrobii Officinalis Caulis（1.5,3 g·kg-1） by gavage,respectively.They were sacrificed after treated with drugs for 8 weeks continuously,and plasma lipid was determined by enzymatic method,expression of TNF-α and IL-6 was measured by ELISA,aortic plaques formation was stained with sudan IV,aortic lumen of atherosclerotic plaques were stained with oil red O and Hematoxylin-eosin,and expression of TNF-α and IL-6 in aortic arches was detected with immunohistochemical method.Result： Dendrobii Officinalis Caulis could reduce TG,TCHOL,LDL-C levels in serum significantly and reduce expression of TNF-α and IL-6;It also could inhibit or slow down formation of atheromatous plaque in aortic valve and aortic arch atherosclerotic plaque areas of ApoE-/- mice,then decreased expression of TNF-α and IL-6 in aortic arch.Conclusion： Dendrobii Officinalis Caulis could reduce serum lipid content,inhibit expression of TNF-α and IL-6 in serum and aortic of ApoE-/- mice,and then reduced inflammatory reactions and relieved atherosclerotic damage.