肝细胞肝癌组织中Bcl-2核表达和SPARC表达临床意义的探讨

Relationship of Bcl-2 expression with SPARC expression in hepatocellular carcinoma,and their correlation with recurrence,metastasis,and prognosis

目的探讨抗凋亡蛋白Bcl-2和富含半胱氨酸的酸性分泌蛋白(SPARC)在肝细胞肝癌中表达的关联性,及其与复发、转移和预后的关系。方法以石蜡包埋组织切片,免疫组织化学SP法染色检测126例肝细胞肝癌,分别在复发与转移组64例和非复发与转移组62例中分析Bcl-2核表达和SPARC的情况。结果复发与转移组Bcl-2核表达高于无复发与转移组(χ2=8.472,P=0.003),复发转移组SPARC表达高于无复发转移组(χ2=6.872,P=0.014),Bcl-2核表达阳性细胞率与SPARC阳性细胞率存在相关性。Bcl-2核表达组患者生存时间较短,差异具有显著性。结论 Bcl-2核表达阳性患者更易出现复发与转移,且与SPARC过度表达存在关联性,表明Bcl-2入核可能对肿瘤转移的相关生物学功能具有调节作用,可作为评价HCC复发、转移和不良生存预后的候选临床标志。

[ Objective ] To determine the correlation of the expression of the anti-apoptotic protein Bcl-2 with the expression of SPARC in human hepatocellular carcinoma, and their relationship with recurrence, metastasis, and prognosis. [Methods] Paraffin-embedded tissue sections of 126 hepatocellular carcinoma cases were subjected to SP immunohistochemical staining. The nuclear expression of Bcl-2 and SPARC were detected. A total of 64 cases had recurrence and metastasis, and the remaining 64 did not. [ Results ] The nuclear expression of Bcl-2 was higher in cases with recurrence band/or metastasis （χ2=8.472, P =0.003）. Similarly, SPARC expression were higher in cases with recurrence and/or metastasis （χ2=6.872, P =0.014）. The nuclear expression of Bcl-2 was correlated with the ex- pression of SPARC. Patients with positive nuclear expression of Bcl-2 had shorter survival time, and the difference was significant. [ Conclusions ] The nuclear expression of Bcl-2 was significantly correlated with metastasis and SPARC expression. Bcl-2 relocation into the nucleus may regulate metastasis-related proteins, which can be used as a clinical marker for evaluating recurrence, metastasis, and prognosis.