HPLC同时测定苦碟子注射液中单咖啡酰基酒石酸、咖啡酸、绿原酸、阿魏酸、菊苣酸的含量

Simultaneous determination of five organic acids in Kudiezi injection by HPLC

该文目的是建立同时测定苦碟子注射液中5种有机酸类成分（单咖啡酰基酒石酸、咖啡酸、绿原酸、阿魏酸、菊苣酸）含量的HPLC分析方法。采用DiamonsilC18色谱柱（4．6mm×200mm，5μm），流动相乙腈一水，梯度洗脱，流速1．0mL·min-1，柱温35℃，检测波长325nm。单咖啡酰基酒石酸、咖啡酸、绿原酸、阿魏酸、菊苣酸在0．64～81．60，0．09—11．10，0．09～11．30，0．10～12．80，0．43～55．50mg·L。线性关系良好，r分别为0．9999，0．9998，0．9998，0．9999，0．9998，平均回收率分别为101．8％，100．9％，99．24％，99．83％，101．9％，RSD不大于2．0％。该方法简单方便、重复性好、准确可靠，可为苦碟子注射液质量控制提供依据。

The aim was to develop a high performance liquid chromatography method for simuhaneous determination of five organic acids in Kudiezi injection. The Diamonsil C18 column （4.6 mm ×200 mm, 5μm） was adopted with acetonitrile and water as the mobile phase at a gradient mode program. The flow rate was 1.0 mL·min-1, detection wavelength was 325 nm, and column tempera- ture was 35℃. The linear range of monucaffeyhartaric acid, chlorogenic acid, caffeic acid, ferulic acid, and chicoric acid were 0. 64- 81.60 （r =0.999 9） ,0.09-11.10 （r=0.999 8） ,0.09-11.30 （r=0.999 8）,0.10-12.80 （r=0.999 9）,0.43-55.50 mg ·L-l（r = 0.999 8）, respectively. The average recoveries were 101.8%, 100.9% ,99.24% ,99.83%, 101.9%, respectively, with RSD of less than 2.0%. The developed HPLC method was simple, sensitive and accurate with good repeatability. This work provided helpful infor- mation for comprehensive quality control of Kudiezi injection.