摘要
目的 观察大麻受体激动剂花生四烯酸乙醇胺(AEA)人U251胶质瘤细胞黏附、趋化运动及侵袭生物学行为的抑制作用.方法 以5μmol/L AEA 12 h和24 h的预处理U251细胞,应用噻唑蓝(MTT)比色法测定U251细胞黏附作用的影响,应用Transwell小室实验检测U251细胞趋化运动和侵袭能力的影响.结果 5μmol/L AEA具有时间依赖性抑制U251细胞黏附、趋化运动及侵袭生物学行为的作用;预处理12 h,U251细胞黏附、趋化运动能力和侵袭能力分别降低45.14%、45.24%和41.86%;预处理24 h后,3种能力分别下降66.53%、81.08%和83.85%.结论 AEA具有对人U251胶质瘤细胞黏附、趋化运动及侵袭生物学行为的抑制作用,且具有时间相关性.
Objective To investigate the effects of the cell adhesion,chemotactic motility and invasion in U251 glioma cell by anandamide (AEA) treatment.Methods Human U251 glioma cell line were cultured with DMEM's medium in vitro.Cultured cells were divided into control group,and 5 μmol/L AEA groups for 12 h and 24 h.The cell adhesion rate of U251 cell was investigated by methyl thiazol tetrazolium (MTT) assay at different times of AEA treatment.The abilities of chemotactic motility and invasion were detected by Transwell.Results MTF showed that AEA inhibited cell adhesion and the rate increased with increasing treatment time of 5 μmol/L AEA.The inhibitive rates were respectively 45.14% for 12 h (P < 0.05) and 66.53% for 24 h (P < 0.01).The abilities of chemotactic motility and invasion also were depressed respectively 45.24% and 41.86% for 12 h (P < 0.05).Those rates were 81.08% and 83.85% for 24h (P <0.01).Conclusion AEA could inhibit the abilities of cell adhesion,chemotactic motility and invasion on human U251 glioma cell line,and this effect has the time dependence.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第9期1810-1812,共3页
Chinese Journal of Experimental Surgery
基金
湖北省科技厅自然科学基金资助项目(2011CDB514)