大鼠颅脑损伤后早期癫痫发作时海马改变

Observation of early seizure and hippocampus change in rats with traumatic brain injury

目的 制作大鼠颅脑液压冲击伤后早期癫痫发作模型,观察海马的胶质纤维酸性蛋白（GFAP）和海马苔状纤维变化.方法 将大鼠随机分组,实验组按照不同的打击力度制作颅脑液压冲击伤模型,测定伤后7d大鼠海马胶质纤维酸性蛋白（GFAP）的表达,行Timm染色,镜下观察.结果 重度冲击伤组大鼠均出现了早期癫痫发作,GFAP平均积分吸光度（A）值：CA3区为38.87±1.24,齿状回为42.35±1.40,苔藓纤维Timm染色颗粒的平均积分A值：1.38 ±0.10,与对照组比较,差异有统计学意义（P＜0.05）.结论 海马区域GFAP表达和苔状纤维出芽增生在伤后早期癫痫发作大鼠明显增加,与癫痫发作敏感性密切相关.

Objective To make model of early seizures after fluid percussion injury in brain of rats,to observe the expression of hippocampal glial fibrillary acidic protein （GFAP） and hippocampal mossy fibers,and to explore the early post-traumatic mechanism of epilepsy seizures.Methods Adult rats were divided into different groups.Experimental group was divided into three different sub-groups and fluid percussion brain injury model was made.GFAP in hippocampal area was detected on the 7th day after injury.Timm staining was carried out and observed under a microscope.Results Early seizures appeared in severe shock group.Average integral optical density of GFAP in the hippocampal region was 38.87 ± 1.24,and average integral optical density of Timm＇s staining granules was 1.38 ±0.10.As compared with control group,the difference was statistically significant （P ＜ 0.05）.Conclusion The GFAP expression in the hippocampal region and mossy fiber spouting hyperplasia is significantly increased in rats with early seizures after injury,and closely related to the sensitivity of epileptic seizure.