左归丸对慢性脑缺血大鼠模型氧化应激损伤的保护作用及其机制

Protective effects of zuogui pill on oxidative stress in rats with chronic cerebral ischemia

目的 探讨左归丸对慢性脑缺血大鼠模型的保护作用及其机制.方法 所有大鼠随机分为对照组、假手术组、慢性脑缺血组、左归丸治疗组.后2组大鼠采用双侧颈总动脉结扎法建立慢性脑缺血大鼠模型.左归丸治疗组术后3周开始每天对大鼠进行左归丸液灌胃,剂量为每天4.0 g/kg,最后得到每组8只大鼠.术后6周检测各组大鼠水迷宫实验潜伏期变化、脑皮层线粒体活性氧（ROS）水平、线粒体的呼吸功能.结果 与慢性脑缺血组比较,左归丸治疗组大鼠水迷宫实验潜伏期缩短[（36.43 ±6.51）s比（43.46±4.28）s,P＜0.05],线粒体ROS水平明显降低（120.52±10.80比137.46±15.28,P＜0.05）,呼吸控制指数和磷氧比值均明显降低（P＜0.05）.结论 左归丸治疗可以改善脑组织线粒体功能,减少ROS的生成,从而减轻慢性脑缺血大鼠的大鼠的氧化应激损伤程度.

Objective To investigate the protective effects of Zuogui Pill on oxidative stress in rats with chronic cerebral ischemia.Methods All rats were divided into control,sham operated,chronic cerebral ischemia and Zuogui Pill treatment group.Rats in later two groups were subjected to permanent bilateral common carotid artery occlusion.At 3 weeks after operation Zuogui Pill was begin to administered orally by gavage to Zuogui Pill treatment group rats at doses of 4.0 g/kg per day.finally 8 rats were in each group.At 6 weeks after operation All rats were tested by water-maze.Reactive oxygen species （ROS） production and respiratory function in rat brain mitochondria was monitored.Results Compared with chronic cerebral ischemia group,rats in Zuogui Pill treatment group had shorter escape latency [（36.43 ± 6.51）vs.（43.46 ±4.28） s,P ＜ 0.05],decreased ROS level （120.52 ± 10.80 vs.137.46 ± 15.28,P ＜0.05）,lower levels of respiration control rate and oxidative phosphorylation parameters （P ＜ 0.05）.Conclusion Zuogui Pill could improve mitochondrial respiratory function and decrease elevated level of ROS,which may be the mechanism of its protection against Oxidative Stress in Rats with Chronic Cerebral Ischemia.