摘要
目的建立酶联免疫间接快速测定鲜乳中β-内酰胺酶的方法。方法应用氨苄青霉素抗体,人工制备氨苄青霉素和辣根过氧化物酶的结合物Amp-HRP,建立氨苄青霉素酶联免疫检测方法。应用该方法分别检测样品反应管和阴性对照管中氨苄青霉素,通过比较两者OD450值,间接判断样品中是否含有β-内酰胺酶。结果成功建立了酶联免疫间接快速测定鲜乳中β-内酰胺酶的方法,检测灵敏度可达0.0005 IU/ml。结论该方法检测结果准确可靠,可广泛应用于鲜乳中β-内酰胺酶的检测。
Objective To establish an indirect ELISA method of β-lactamase detection in milk.Methods The Elisa method of Ampicillin was set up and optimized with ampicillin-HRP conjugate and Ampicillin antibody.The method was used to detect the ampicillin in samples and negative control,and β-lactamase was determined indirectly by comparing OD450 values.Results The indirect ELISA method for β-lactamase was established,and the sensitivity was 0.0005 IU/ml.Conclusion This method was accurate and reliable,and could be widely applied to detect β-lactamase in milk.
出处
《中国食品卫生杂志》
北大核心
2013年第5期405-409,共5页
Chinese Journal of Food Hygiene
基金
浙江省质监系统科研计划项目(20100401)
