高效液相色谱法同时测定植物油中的15种多环芳烃

Simultaneous determination of fifteen polycyclic aromatic hydrocarbons(PAHs)in plant oil by high performance liquid chromatography

目的建立高效液相色谱-荧光法测定植物油中的15种多环芳烃。方法样品用正己烷溶解,过中性氧化铝固相萃取小柱净化,采用Waters-PAHs专用柱(4.6 mm×250 mm,5μm)分离,乙腈-水梯度洗脱,流速1.5 ml/min,柱温30℃,进样量10μl;荧光检测器检测,外标法定量。结果方法检出限在0.15～2.0μg/kg之间,样品加标回收率在78.0%～115.2%之间,RSD在0.2%～2.0%之间。结论该方法具有前处理简单、灵敏度高、重复性好等优点,可用于植物油中15种多环芳烃的含量测定。

Objective To establish a method for simultaneous determination of 15 PAHs in plant oil by high performance liquid chromatography with fluorescence detection.Methods The sample was dissolved in n-hexaneand,and cleaned up with neutral alumina SPE cartridges.The separation of 15 PAHs was carried out by waters-PAHs column(4.6 mm×250 mm,5 μm) with a gradient elution using acetonitrile-water as mobile phase at a flow rate of 1.5 ml/min,the column temperature was 30 ℃,and the injection volume was 10 μl.Detection was carried out by a fluorescence detector with external standard.Results The limit of detection(LOD) was in the range of 0.15~2.0 μg/kg with average recovery ranging from 78.0% to 115.2%.The relative standard deviation was in the range of 0.2%~2.0%.Conclusion The method had the advantages of simple pretreatment,high sensitivity and reproducibility,which could be applied to the determination of the 15 PAHs in plant oil.