液体发酵生产猪苓胞内多糖的条件优化

Optimization of Liquid Fermentative Conditions for Intracellular Polysaccharides from Polyporus umbellatus

目的:优选猪苓液体发酵生产胞内多糖的工艺条件。方法:采用摇瓶培养法制备猪苓胞内多糖的发酵培养基,以猪苓胞内多糖得率为指标,通过单因素试验考察氮源、碳源和发酵条件,正交试验优选培养基的组成。结果:液体发酵生产猪苓胞内多糖的最适碳源为蔗糖,最适氮源为黄豆粉,优选的培养基组成为马铃薯10.0%,蔗糖4.0%,黄豆粉0.3%,KH2PO40.2%,MgSO4·7H2O 0.15%;发酵条件为初始pH 5.2～5.6,振荡速度180～200 r·min-1,培养温度28℃,培养时间7 d。结论:液体发酵猪苓胞内多糖具有周期短、产量高的优势,对猪苓多糖工业化生产和降低生产成本具有重要意义。

Objective： To optimize liquid fermentative conditions of intracellular polysaccharides from Polyporus umbellatus. Method： Fermentative medium for intracellular polysaccharides in P. umbellatus was prepared by shake flask cultivation. With yield of intracellular polysaccharides from P. umbellatus as index, nitrogen source, carbon source and fermentation conditions were investigated by single factor tests, orthogonal test was adopted to optimize composition of culture medium. Result： The best carbon sources was saccharose, optimum nitrogen source was soybean meal, optimized composition of culture medium was as following： potato 10.0% , saeeharose 4.0% , soybean meal 0.3% , KH2PO4 0.2% , MgSO4·7H2O 0. 15% ; Fermentation conditions were.： initial pH 5.2-5.6, shaking speed 180-200 r ·min^-1, culture temperature 28 ℃, culture time 7 d. Conclusion： It had advantages in fermentation period and polysaccharide production by liquid fermentation, meanwhile it was important to reduce costs and industrial production of polysaccharides from P. umbellatus.