大孔树脂吸附纯化维药天山堇菜总黄酮

Purification of Flavonoids from Viola tianshanica by Macroporous Resin

目的:研究大孔树脂吸附纯化天山堇菜总黄酮的条件及参数,为黄酮类化合物的富集提供参考。方法:比较了3种大孔树脂对天山堇菜总黄酮的吸附性能;以天山堇菜总黄酮的得率和纯度为考察指标,对大孔树脂吸附纯化天山堇菜总黄酮的工艺进行筛选。结果:AB-8大孔树脂对天山堇菜总黄酮的吸附性能最好,达48.0 mg·g-1湿树脂,AB-8大孔树脂对天山堇菜总黄酮最佳洗脱浓度为35%乙醇,洗脱液为4 BV。天山堇菜总黄酮的得率达2.08%,纯度达71.1%。结论:大孔树脂富集黄酮类化合物工艺简便,树脂再生容易,可用于工业化生产。

Objective： To investigate the condition and parameters of adsorption and purification of total flavones contained in Viola tianshanica by macroporous resin so as to provide methods for the enrichment of flavonoids. Method： The adsorption properties of 3 kinds of macroporous resins for total flavonoids of V. tianshanica were compared; the yield and purity of total flavonoids from V. tianshanica were considered as standards, the purification process of total flavonoids in V. tianshanica by macroporous resin was selected. Result： It was found that theadsorption properties of AB-8 macroporous resins for total flavonoids of V. tianshanica were the best, up to 48 mg ·g^-1 wet resin, the best conditions to elute the total flavonoids adsorbed by AB-8 macroporous resin was 35% ethanol, resin vs. crude drug was 2.＇5：1, ratio of diameter to height was 1：8. Using this process, the yield of the total flavones of V. tianshanica was up to 2.08%, the purity .was 71.1%. Conclusion： This process is simple and practicable, it can be used to purify the total flavones contained in V. tianshanica.