玉叶金花提取液对动物模型抗炎抑菌作用研究

Anti-inflammatory and Anti-bacterial Activities of Extractsfrom Mussaenda parviflora Extract in Animal Models

目的:选择玉叶金花提取液对动物模型进行抗炎、抑菌作用研究分析。方法:将小鼠随机分为对照组、阳性对照组和玉叶金花提取液高、中、低剂量组(24,12,6 g·kg-1)灌胃给药,采用二甲苯致小鼠耳廓肿胀、棉球肉芽肿、角叉菜胶致大鼠足跖肿胀等炎症模型,以及体外抑菌实验,评价玉叶金花提取液的抗炎、抑菌作用。结果:玉叶金花提取液明显抑制小鼠耳廓肿胀(P<0.05)、大鼠棉球肉芽肿以及大鼠足跖肿胀的形成(P<0.05);玉叶金花提取液对多种致病菌均有明显的抑制作用(P<0.05)。其半数抑菌浓度(MIC50)金黄色葡萄球菌0.95μg·mL-1、大肠埃希菌0.75μg·mL-1、肺炎球菌0.65μg·mL-1、链球菌0.50μg·mL-1、痢疾杆菌0.25μg·mL-1。结论:玉叶金花提取液对急慢性炎症有明显的抑制及体外抑菌作用。

Objective： To select Mussaenda extract on animal models of inflammatory, antibacterial research and analysis. Method： The mice were randomly divided into control group, positive control group and M. parviflora extract high, medium and low dose group （24, 12, 6 g ·kg^-1） administered orally, using xylene- induced mouse ear profile swelling, granuloma; carrageenan-induced rat paw edema inflammation model, and antibacterial experiments in vitro evaluation M. parviflora extract anti-inflammatory, effect. Result： M. parviflora extract significantly inhibited mouse ear edema （P 〈 0.05） , as well as rat granuloma rat paw edema formation （P 〈 0. 05） ; M. parviflora extract on a variety of pathogenic bacteria were inhibited （ P 〈 0.05 ）, the minimum inhibitory concentration （MICs0） Staphylococcus aureus 0.95 μg·mL^-1, E. coli 0.75 μg·mL^-1, pneumococcal 0.65μg·mL^-1, Streptococcus 0. 50 μg·mL^-1, Shigella 0.25 μg·mL^-1. Conclusion： M. parviflora extract on acute and chronic inflammation and in vitro inhibition significantly inhibitory effect.