产蛋白酶深海细菌的筛选及其蛋白酶酶学性质

Isolation of producing-proteases marine bacteria and characterization of the proteases in fermented liquid

从中国南海深海海泥中筛选出了2株产中低温碱性蛋白酶的菌株SWJS33,SWJS22,并对其发酵液中的蛋白酶性质作了初步研究。研究结果显示,SWJS33,SWJS22所产蛋白酶适用于较广的pH及温度范围,且有较好的温度稳定性和耐盐性。采用16S rDNA结合细菌常规鉴定方法,确认分离得到的SWJS33,SWJS22分别为地衣芽孢杆菌(Bacillus licheniformis)和解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。

Two protease-producing marine bacterial strains, named SWJS33 and SWJS22, were isolated from samples of deep sea mud from the South China Sea. The protease in the fermented liquid of these isolate strains was researched. The results showed that proteases which produce by the strains of SWJS33 and SWJS22 could be used in wide ranges of pH and temperature. And they not only have good temperature stability, but also have good salt resist- ances. Based on the physiological and biochemical characteristics and analysis of 16S rDNA sequence of SWJS33 and WSJS22, the two strains were characterized and identified as species of Bacillus licheniformis and Bacillus amylolique- faciens. And a new way is expected to the development and utilization of deep-sea microbial protease.