罗非鱼鱼鳞明胶微胶囊化抗坏血酸的研究

Study on ascorbic acid micro-encapsulation by tilapia scales gelatin

以罗非鱼鱼鳞明胶为壁材,玉米油为流动相,山梨醇酐三硬脂酸酯(司盘65)为乳化剂,结合超声波技术,制备包埋抗坏血酸的微胶囊。探讨了乳化剂用量,固化时间,流动相用量,鱼鳞明胶与抗坏血酸质量比对微胶囊包埋率的影响。结果表明:1 mL含质量比为2∶1的15 mg/mL的明胶与抗坏血酸的混合液,乳化剂用量为100μL,玉米油用量为20 mL,在40℃下以0.12 g/mL谷氨酰胺转胺酶(TG酶)固化7 h,微胶囊包埋率达到89.78%,同样条件下获得的动物源明胶制备的微胶囊包埋率仅为57.3%,且该微胶囊粒径分布较为集中,平均粒径为3.57μm,证明了鱼鳞明胶对哺乳动物源明胶具有一定的可替代性。

Tilapia scale gelatin was used as wall material, corn oil was mobile phase, sorbitan tristearate （Span 65） was emulsifier, ultrasonic technology was applied to prepare ascorbic acid microcapsules. The effects of emul- sion, mobile phase, and ratio of gelatin and ascorbic acid were discussed. The results showed that the best parameters were： lmL mixed solution at the mass ratio 15mg/mL gelatin and ascorbic acid with 2： 1, 100 μL emulsion, 20 mL corn oil, 0. 12 g/mL transglutaminase react at 40 ℃ for 7 hours. Under the above condition, the obtained microcap- sules had an average particle diameter of 3.57μm. The encapsulation efficiency was 89.78% , higher than mammali- an gelatin encapsulate of 57.3%. This indicated that fish scale gelatin can replace mammalian gelatin.