益髓生血颗粒对辐射损伤小鼠造血祖细胞增殖功能的影响

Effect of Yisui Shengxue Granules on the Proliferation of Hematopoietic Progenitor Cells in Irradiated Mice

目的研究益髓生血颗粒治疗地中海贫血的可能作用机制。方法 72只小鼠随机分为正常对照组、模型对照组、阳性对照组和益髓生血颗粒高、中、低剂量组,每组12只。除正常对照组外,其余各组采用60 Co-γ射线3.5Gy全身一次性照射,造成小鼠骨髓造血抑制模型。益髓生血颗粒高、中、低剂量组分别给予益髓生血颗粒10.0g/kg,5.0g/kg,2.5g/kg,灌胃给药后2d进行造模,之后再连续给药7d;阳性对照组造模后皮下注射重组人粒细胞刺激因子注射液30μg/kg,每天1次,连续7d;正常对照组、模型对照组给予等量蒸馏水灌胃。在造模后3、7d进行骨髓造血祖细胞集落培养,计数红系集落生成单位(CFU-E)、红系爆式集落生成单位(BFU-E)、混合集落生成单位(CFU-Mix)、粒单系集落生成单位(CFU-GM)、巨核系集落生成单位(CFU-Meg),造模后7d进行CD34+细胞检测。结果与正常对照组比较,模型对照组于造模后3、7d各集落生产单位明显降低(P<0.01)。与模型对照组比较,阳性对照组和益髓生血各剂量组在3、7d均促进CFU-GM、CFU-E增殖(P<0.05或P<0.01);益髓生血颗粒高、中剂量组在3d时提高CFU-Meg方面优于阳性对照组和益髓生血颗粒低剂量组(P<0.05);益髓生血颗粒高、中剂量组7d时在提高CFU-E方面优于阳性对照组和益髓生血颗粒低剂量组(P<0.05)。模型对照组CD34+细胞数较正常对照组明显下降(P<0.01),阳性对照组和益髓生血颗粒低剂量组CD34+细胞数较模型对照组显著上升(P<0.05)。结论益髓生血颗粒能促进小鼠骨髓造血祖细胞增殖,促进骨髓有效造血,并且以高、中剂量作用显著。

Objective To research the possible mechanism of Yisui Shengxue Granules （granules to boost marrow and engender blood, YSG） for thalassemia. Methods Seventy two mice were randomized into the normal control group, model control group, positive control group and YSG high-dose, middle-dose and low-dose groups, with 12 in each. The bone marrow suppression mice models were established with 60Co y-ray 3.5Gy disposable body irradiation. YSG high-dose, middle-dose and low-dose groups were gavaged with YSG containing 10. 0g/kg 5. 0 g/kg and 2. 5 g/kg crude drug. The models were established after 2-day intragastric administration and then administered for 7 consecutive days. The positive control group was injected with 30 t^g/kg ~ recombinant human granulocyte colony-stimulating factor once daily for 7 days. The normal control group and model control group were gavaged with equal amount of distilled water. The bone marrow hematopoietic progenitor cell colony was cultured on the 3rd and 7th day after modeling. The colony-forming unit-erythroid （CFU-E）, burst-forming unit-erythroid （BFU-E）, mixed colony-forming unit （CFU-Mix）, colony-forming unit-granulocyte/macrophage （CFU-GM） and colony-forming unit-megakaryocytes （CFU-Meg） was counted. The CD34＋ cell was detected on the 7th day after radiation. Results Comparing with the normal control group, the colony-forming units were significantly decreased in the model control group on the 3rd and 7th day after modeling. Comparing with the model control group, the CFU-GM and CFU-E proliferated significantly in the positive control group and YSG groups on the 3rd and 7th day （P〈0.05 or P〈0.01）. Comparing with the positive control group and YSG low-dose group, the CFU-Meg proliferated significantly in YSG hige-dose and middle-dose groups on the 3rd day （P〈 0.05）. Comparing with the positive control group and YSG low-dose group, the CFU-E proliferated significantly in YSG hige-dose and middle-dose groups on the 7th day （P〈0.05）. The CD34＋ cell count in the model control group was significantly lower than that in the normal control group （P〈0.01）. Comparing with the model control group, the CD34＋ cell counts in the positive control group and YSG low-dose group were significantly increased （P〈 0, 05）. Conclusion YSG can improve the proliferation of hone marrow hematopoietic progenitor cells, promote bone marrow effective hematopoietie, especially in high and middle dose groups.