基于响应面法的秸秆与粪便联合制氢预混工艺优化

Optimization of Premixing Process of Joint Hydrogen Production by Straw and Manure Based on Response Surface Method

借助Design-Expert(version 8.0)软件,采用Box-Benhnken试验设计法及响应面分析法,对食草性牲畜粪便与秸秆利用光合微生物进行联合制氢的预混工艺进行优化研究。设定三因素三水平的响应面试验,三因素为pH值、预混温度、预混时间,响应值为OD值,零点试验进行3次。使用Design-Expert软件对响应面试验数据进行回归分析,结果表明,响应面模型中二次方模型表达最准确,三因素间存在显著相关性,通过回归方程的修正得到优化后的预混工艺为:时间6.3 d、pH值4.9、温度48℃,预估计最大OD值为2.696 8,预测值与验证试验平均值接近。

In order to optimize the premixing process of the herbivorous livestock manure and straw for joint photosynthetic microorganisms hydrogen production,the method of Box-Benhnken experimental design and response surface analysis were used with Design-Expert software.The response surface experiments contained three factors,such as pH value,premixed temperature and premixed time.Response value was OD value and zero point experiments proceed three times.The response surface experiment data were analyzed by Design-Expert software,and the results showed that the quadratic model was the most accurate expression.The three factors existed significant correlation.The premixing process was optimized by the amendment of the regression equation： time of 6.3 d,the pH value of 4.9,temperature of 48℃,the estimated maximum OD value of 2.696 8.The predicted value was closed to the average value of the validation test.The results provided a theoretical basis for the experimental study of photosynthetic microorganism hydrogen production.