胚胎干细胞在裸鼠眼内向神经元和视网膜样结构分化

Differentiation of embryonic stem cells into neurons and retina-like structure in nude mice

目的 研究小鼠胚胎干细胞在裸鼠眼内的生长特性。 方法 将未分化的胚胎干细胞移植到Balb/ c裸小鼠眼内。14～ 2 0 d处死裸鼠 ,观察其形态学和免疫组织化学变化。 结果 胚胎干细胞移植到裸鼠眼内 ,在眼前房和玻璃体腔内能见到黄白色物 ,逐渐增大。光镜检查 :在前房和玻璃体腔内均能见到未分化细胞和已分化的细胞 ,部分细胞的形态和排列方式与视网膜组织中的一些结构相似 ,其免疫组织化学抗神经元特异性烯醇化酶染色大部分已分化细胞呈强阳性反应。 结论 胚胎干细胞在裸小鼠眼内的特定环境中 ,向神经元和视网膜样结构分化。

Purpose [WT5”BZ]To investigate the characteristics of intraocular growth of mice embryonic stem cells (ESC) in nude mice. [WT5”HZ]Methods [WT5”BZ]The undifferentiated murine ESC in vitro were transplanted into the eyes of nude mice.Mophological and immunohistochemical examinations were implemented. [WT5”HZ]Results [WT5”BZ]Two to three days after transplantation,yellowish white granules and masses were seen inside the anterior chamber and vitreous cavity and enlarged gradually.Morphological examination showed that there were undifferentiated cells and differentiated cells in anterior chamber and vitreous cavity.The morphology and alignment of some differentiated cells were similar to those of the retina of nude mice.The cells were highly positive in NSE staining. [WT5”HZ]Conclusion [WT5”BZ]The transplanted ESC could grow in the eyes of nude mice and differentiate into neurons and retina like structure. [WT5”HZ]