可定量的氧致血管增生性视网膜病变小鼠模型

A quantifying model of vascular proliferation in oxygen-induced retinopathy

目的 建立可对视网膜新生血管进行定量研究的小鼠模型 ,用于视网膜新生血管发生机制的探讨及治疗方法的评价。 方法 给氧箱组 16只 1周龄 C5 7BL / 6小鼠在浓度为 75 %的氧箱中生活 5 d,然后回到正常氧环境中 ;16只同龄小鼠生活在正常氧环境中做对照。墨汁灌注视网膜铺片了解氧所导致的视网膜血管改变 ;计数矢状面 6 μm视网膜切片中突破内界膜的血管内皮细胞 ,反映视网膜血管的增生 ;视网膜切片还做了血管内皮细胞生长因子 (vascular endothelial growth factor,VEGF)和碱性成纤维细胞生长因子 (basic fibroblastgrowth factor,b FGF)免疫组织化学染色。 结果 高氧促视网膜血管收缩、闭塞 ,相对低氧致视网膜血管扩张、增生。突破视网膜内界膜的血管内皮细胞数 ,给氧组平均 2 4个 /切面 ,对照组平均<1个 /切面 ,二者相比差异有显著性的意义 (P<0 .0 0 1)。与对照组相比 ,给氧组小鼠视网膜内层 VEGF的表达明显增强。 结论 该小鼠模型中视网膜新生血管的发生与缺氧相关 ,并可对其定量观察 ,是进行视网膜新生血管发生机制及治疗研究的合适模型。

Purpose [WT5”BZ]To estabalish a quantifying model of retinal neovascularization suitable for the study of pathogenesis and therapeutic intervention for the retinal neovascularization. [WT5”HZ]Methods [WT5”BZ]Sixteen one week old C 57 BL/6 mice were exposed to 75% oxygen for 5 days and then to room air and 16 mice of the same age kept in room air as controls.Ink perfused retinal flatmount was examined to assess the oxygen induced changes of retinal vessels.The proliferated neovascular response was quantitated by counting the nuclei of endothelial cells of new vessels extending from the retina into the vitreous in 6 μm sagittal cross sections. VEGF and bFGF were determined on the cross sections after immunohistochemcal stain. [WT5”HZ]Results [WT5”BZ]Constriction and closure of the blood vessels were found under the hyperoxia condition,and dilation and proliferation were found under the relatively hypoxia status.There was a mean of 24 neovascular nuclei per cross section in the oxygen treated retina and less than 1 nucleus in the control group ([WT5”BX]P<0.001).VEGF stain was found stronger in the inner retinal layer of oxygen treated mouse than in that of the controls. [WT5”HZ]Conclusion [WT5”BZ]The quantifying model of retinal neovascularization may fascilitate the further researches of medical intervention and pathogenesis of retinal neovacularization. [WT5”HZ]