一种数字荧光显微系统及其在单粒子追踪实验中的应用

A Digital Fluorescence Microscope System and Its Application in Single Particle Tracking Experiments

为实现对活细胞表面受体的追踪 ,我们在制冷 CCD的基础上研制了一套高性能数字荧光显微系统。利用这套系统和经过我们改善的单粒子追踪 (SPT)技术 ,根据受体荧光图象呈二维高斯分布的特性 ,使每幅图象中受体位置的匹配精度达到了 2 5nm。经改善的 SPT技术不但可以精确测量受体的光强 ,而且还可以提供高时间分辨力。本系统和方法为单个活细胞显微图象的检测和分析奠定了较好基础。

A high performance digital fluorescence imaging system based on cooled CCD camera is developed for tracing receptor on the surface of living cells.With this system and the improved Single Particle Tracing (SPT) techniques,the matching accuracy for the receptor position of each image has reached 25nm according to the receptor fluorescent image emerging 2 D Gaussian distribution property.The improved SPT techniques not only can accurately measure the light intensity of the receptor,but also provide a high temporal resolution.The system and method lay a better basis for detecting and analyzing the microscopic image of single living cell.