宫内和哺乳期接触多氯联苯118和126对雄性仔鼠生殖功能影响

Effect of in utero and lactational exposure to a binary mixture of 3,3',4,4',5-pentachlorobiphenyl( PCB126) and 2,3',4,4',5-pentachlorobiphenyl( PCB118) on reproductive function in male rat offsprings

目的探讨宫内和哺乳期接触多氯联苯(PCB)118和PCB126混合物对雄性子代大鼠生殖功能的影响。方法根据浙江某地母乳混合样中PCB118和PCB126含量及比例,按10、100和500倍配制成剂量为0.096、0.96和4.8μg TEQ/kg的混合物,在孕15 d对SD大鼠进行一次经口染毒,对母鼠的体重、雄性子代各发育阶段的肛门生殖口距离(AGD)和体重、脏器重量、精子计数及血清中睾酮水平等指标进行评估。结果各剂量组对母鼠没有明显毒性,0.96和4.8μg TEQ/kg组雄性仔鼠出现体重增加迟缓、AGD及精子数减少现象;雄性生殖器官如附睾、前列腺和精囊的重量改变只发生在出生后70日龄(PND70)4.8μg TEQ/kg组和0.96μg TEQ/kg组(附睾);血清睾酮水平没有发生明显变化。结论宫内和哺乳期接触PCB118和PCB126混合物在未观察到母体毒性剂量下能延迟子代雄性大鼠生长发育进程,并对其生殖系统产生损害。

Objective To examine the effects of in utero and lactational exposure to a binary mixture of 3,3＇, 4,4＇, 5-pentachlorobiphenyl （PCB126） and 2, 3＇, 4,4＇, 5- pentachlorobiphenyl （PCB118） on reproductive function in male rat offsprings. Methods Pregnant Sprague-Dawley rats were given the mixture by gavage with 0. 096, 0.96, 4. 8 μg toxic equivalency （TEQ）/kg on gestational day （GD） 15. The effects of mixture on F1 rats were observed. Results The male offspring in the 0.96 and 4. 8 μg TEQ/kg groups showed significant inhibition of body weight gain, anogenital distance （AGD） and sperm counts. Mixtures at 4.8 μg TEQ/kg significantly reduced weights of accessory sex organs, but only prostate weight reduced at 0. 96 μg TEQ/kg. There were no significant differences on serum testosterone. Conclusion The results demonstrated that in utero and lactational exposure to the mixtures of PCB126 and PCB118 affected development of male offspring and impaired adult male offspring reproductive organs.