高效液相色谱法快速测定水中苦味酸

Quickly Determination of Pricia Acid in Water By High Performance Liquid Chromatography

采用高效液相色谱法,研究了水中苦味酸的快速测定方法,优化了液相色谱的各项分析条件。结果表明,流动相为甲醇∶水(0.01%三氯乙酸)(体积比45∶55)、柱温40℃、检测波长350 nm为最佳的检测条件,在最佳检测条件下,苦味酸的检出限为0.4μg/L。地表水和地下水高、中、低3种浓度的加标回收率均在88%以上,相对标准偏差(RSD)均小于5%,污水的加标回收率为71%～90%,RSD为7.9%。

Pricia acid in water was determined by high performance liquid chromatography.The analytical conditions of HPLC were optimized though a series of tests.Results showed the optimum detected conditions were methanol-water（0.01%TFA） volume ratio of 45∶55 as the mobile phase and the temperature of 40 ℃.The detection wavelength was 350 nm for ultraviolet detector.Under the optimized conditions,limits of detection of this method was 0.4 μg/L.The recovery tests were analyzed using this new method： Three different grade include high、middle and low concentrations were added in surface and underground water samples separately,recoveries results of three concentrations were all above 88%,with relative standard deviations（RSD） blow 5%;And waste water recoveries were between 71% and 90%,with RSD was 7.9%.