津蓟咽炎Ⅰ号合剂定性定量研究

Qualitative and quantitative method of Jinji Yanyan No.1 mixture

目的建立中药合剂津蓟咽炎I号的质量控制方法。方法采用薄层色谱法对合剂中金银花所含绿原酸和甘草中所含甘草酸单胺盐进行定性研究；以高效液相色谱法对合剂中含有的腺苷、黄芩苷进行定量研究。结果薄层色谱斑点清晰，阴性样品无干扰；检测腺苷的流动相为0．04mol·L^-1NaH2PO4-乙腈（94：6），流速为1．0mL·min，检测波长为260nm；检测黄芩苷的流动相为甲醇．水（55：45），10％磷酸调整pH至3．3，检测波长280nm，流速1．0mL·min^-1。腺苷进样量在4．5～54．0μg·mL^-1与峰面积呈良好的线性关系，r=0．9997，平均回收率为101．1％，RSD为0．97％（n=9）；黄芩苷进样量在10～70μg·mL^-1与峰面积呈良好线性关系，r=0．9996，平均回收率为102．0％，RSD为0．88％（n=9）。结论本方法简便、准确、灵敏度高、专属性和重现性好，可作为该产品的质量控制方法。

Objective To establish a method to control the quality of Jinji Yanyan No.1 mixture. Methods Glycyrrhizin in glycyrrhiza uralensis fisch, and chlorogenic acid in onicera japonica thunb were authenticated by thin-layer chromatography. An HPLC method for the determination of adenosine and baicalin in Jinji Yanyan Nod mixture was established. Results The spots on the TLC plate were clear. The negative sample did not interfere. Adenosine was determined with 0.04 tool ~ L- ~NaHzPO4-acetonitrile （94 ： 6） as the mobile phase, the flow rate was 1.0 mL ~ min t and the detection wave- length was 260 nm. Baicalin was determined with methanol-water （55 ： 45） as the mobile phase which was adjusted to pH 3.3 with 10% H3PO4, The flow rate was 1.0 mL . min^ - 1 and the detection wavelength was 280 nm. Good linearity was obtained at 4.5 -- 54.0 μg . mL^-1 （r = 0.999 7） for adenosine and 10 - 70 μg.mL^-1 （r = 0.999 6） for baicalin. The average recovery was 101.1% （RSD = 0.97%, n = 9） for adenosine and 102.0% （RSD = 0.88%, n = 9） for baicalin. Conclusion The method is specific, accurate and reproducible, and can be used for the quality control of Jinji Yanyan No. 1 mixture.