摘要
从甘南州玛曲县高寒草甸土壤中筛选出一株低温纤维素降解菌M7。通过形态观察、生理生化及16SrRNA序列比对分析,发现菌株M7与Bacillus subtili strain XS6进化距离最近,初步鉴定为枯草芽孢杆菌(Bacillus subtili)。通过Box-Benhnken中心组合设计和响应面分析法(RSM),对其生长和产羧甲基纤维素酶(CMCase)条件进行优化。结果表明:温度20℃,培养基初始pH5.0,转速175r/min,酵母膏2.5g/L和微晶纤维素2.5g/L,蛋白胨5g/L,菌株M7所产CMCase达到最大值。
A strain M？ with cellulose degradation ability was screened from alpine meadow soil of Maqu county in Gansu province.It is identified as Bacillus subtilis by morphology, physiological and biochemical property and ]6SrRNA sequence analysis.The initial study on its producing cellulase showed that the temperature was 20℃, initial pHS.0, revolving speed l？Sr/min, peptone 5g/L, yeast extract 2.Sg/L and microcrystalline cellulose 2.5g/L. After the optimization of Box-Benhnken design and response surface methodology,the cellulase activity reached the maximum.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第19期127-130,134,共5页
Science and Technology of Food Industry
基金
甘肃省自然科学基金(0710RJZA059)
教育部全国大学生创新性实验计划项目(224002)
关键词
羧甲基纤维素酶
响应面
低温细菌
高寒草甸
优化
carboxymethyl cellulose （CMCase）
response surface methodology
cold- adapted bacterium
alpinemeadow
optimization
