碱法制备米蛋白过程中微波辅助降解黄曲霉毒素B_1的研究

Study on degradation of aflatoxin B_1 during the preparation of rice protein by microwave-assisted alkali treatment

针对稻米中所存在的黄曲霉毒素污染的问题,本文研究了碱法制备米蛋白过程中微波辅助处理对黄曲霉毒素B1(AFB1)降解率的影响。结果表明,碱法制备米蛋白工艺可使AFB1浓度下降46.83%,米蛋白中AFB1残留浓度为33.91μg/kg,不符合国家标准(≤10μg/kg);基于该工艺研究,单位体积微波功率750W/L和微波处理时间7min为微波辅助碱法降解AFB1的最佳处理条件,AFB1降解率为88.51%,米蛋白中AFB1残留浓度为7.33μg/kg,符合国家标准。同时,AFB1初始浓度对微波辅助碱法降解AFB1的影响不显著,通过对微波辅助碱法降解AFB1工艺进行动力学模型拟合,表明该降解特征符合一级动力学模型。

Since the problem of afiatoxin contamination in rice might occur, the degradation of AFB1 during the preparation of rice protein by the microwave-assisted alkali treatment was investigated in this study.The results showed that the concentration of AFB1 was decreased 46.83% during the preparation of rice protein by the alkali treatment,and the residual concentration of AFB1 was 33.91μg/kg,which did not reach the requirement of national standard （ ≤ 10μg/kg）.And based on the alkali treatment,the optimal microwave-assisted condition of degradation of AFB1 was microwave power per unit volume 750W/L and microwave treating time 7min.Under this condition,the degradation rate of AFB, was 88.51%, and the residual concentration of AFB1 was 7.33μg/kg.Furthermore, the effect of initial concentration of AFB1 on degradation of AFBI by the microwave-assisted alkali treatment was not significant.And through the kinetic model fitting of degradation of AFB1 by the microwave-assisted alkali treatment, it showed that the degradation characteristic of AFB1 was in line with the first order dynamic model.