胶体金免疫层析法快速检测食品中庆大霉素残留

Development of a one-step immunochromatographic strip test for rapid detection of gentamicin residue in food

应用胶体金免疫层析技术,建立一种快速检测食品中庆大霉素残留的方法。采用免疫竞争法,柠檬酸三钠还原制备胶体金颗粒,标记抗庆大霉素单克隆抗体并喷于玻璃纤维上,庆大霉素偶联抗原和羊抗兔二抗分别包被在硝酸纤维素薄膜表面作为检测线(T线)和控制线(C线),制备检测庆大霉素的胶体金免疫层析试纸条,并对试纸条的灵敏度、特异性、准确性和稳定性进行测定。测试结果表明:庆大霉素快速检测试纸条的灵敏度为0.7μg/mL,检测时间为10min,稳定性和特异性良好,与其他同类物质无交叉反应,其检测结果与酶联免疫吸附测定法(ELISA)检测结果呈现了很好的一致性。该方法快速简便、灵敏度高、特异性强、准确性和稳定性好,非常适合现场快速检测庆大霉素。

A gold immunochromatography assay（GICA）was studied to establish a rapid assay for detection of Gentamicin（GM）.Colloidal gold marked coupled with monoclonal antibody against GM was jet sprayed onto the glass fiber.Gentamicin- BSA and sheep- anti- rabbit immunoglobulins dispersed on a nitrocellulose （NC） membrane to be the test line（T）and control line（C）.Gold immunochromatographic strips which allowed to specifically detect GM were obtained and its sensitivity, specificity, accuracy and stability were evaluated in this study.The results showed that the sensitivity of rapid assay of Gentamicin was 0.7 μg/mL and detection time within 10min.The assay showed good stability and specificity, and no cross-reactivity with other analogs of GM.A good consistency was observed between results of GM determination by this method and ELISA.This method was rapid, easy,sensitive,specific,accurate and stable, so it was very simple and convenient to rapidly detect GM on the spot.