雪里蕻非特异脂质转运蛋白基因的克隆与分析

Isolation and Molecular Characterization of nsLTP-Like Gene from Brassica juncea var. multiceps( Cruciferae)

非特异脂质转运蛋白是植物生命活动中一类重要的活性蛋白,这类蛋白在植物的抗性和防御中行使着重要的功能。近年来研究发现这类蛋白还与植物的有性生殖密切相关。通过已得到的普通白菜的脂质转运蛋白基因BcMF15的核苷酸序列,在其基因全长两侧设计引物,从雪里蕻中克隆得到该类活性蛋白基因,命名为BjLTP(登陆号:EU082009)。该基因全长650 bp,不含内含子。不同组织的表达特征分析发现,BjLTP在雪里蕻的花蕾、开放的花中特异表达,推测BjLTP可能与花粉的发育有关。蛋白质特征预测及蛋白序列结构分析发现BjLTP是一个跨膜蛋白,具有显著的疏水区。序列同源比对表明该基因与白花芥蓝、拟南芥等的脂质转运蛋白基因有很高的相似性,证明BjLTP是LTP家族的成员之一。

Non-specific Lipid transfer proteins （nsLTPs） were a group of important active proteins in in higher plants. Their function has been suggested to have a role in plant resistance and defense response. According to re- cent studies, LTPs play an important role in plant reproduction and development. In this study, BjLTP was cloned from Brassica juncea var. multiceps based on BcMF15 from Brassica campestris ssp. chinensis var. communis BjLTP was 650 bp （ EU082009 ） in length with no intron. RT-PCR analysis showed that BjLTP was expressed in flower buds and open flower but not other tissues. The gene may act as nsLTPs involving in pollen development. Protein characteristics and sequence structure were predicted, that it was a transmembrane protein with obvious hydrophobic region. BjLTP was identified as a Lipid transfer protein gene according to its high identity with Brassica oleracea （MF15bh） and Arabidopsis thaliana LTP.