采收期内铁皮石斛多糖类成分含量比较

Comparison of Polysaccharide Contents of Dendrobium officinale in Collection Periods

目的研究采收期内铁皮石斛多糖类成分含量比较。方法利用苯酚一硫酸法测定多糖的含量，高效液相法测定甘露糖的含量及甘露糖与葡萄糖的峰面积比值。结果铁皮石斛多糖含量为26．52％～46．78％，甘露糖含量为13．36％～25．25％，甘露糖与葡萄糖峰面积比值为2．48—5．91。结论不同来源的铁皮石斛中多糖类成分含量有较大差异，但均符合药典标准，其中云南产的多糖类成分含量高于浙江产，2年生及以上样品甘露糖含量高于1年生。

OBJECTIVE To compare the polysaccharide contents of Dendrobium officinale in collection periods. METH- ODS The contents of polysaccharide and mannose were determined by phenol-sulfuric acid method and HPLC. The ratio of peak area between mannose and glucose was worked out by HPLC. RESULTS The polysaccharide content of Dendrobium officinale distributed from 26.52% to 46.78% ,the mannose content was between 13.36% and 25.25% ,and the ratio of peak area be- tween mannose and glucose was 2.48 - 5.91. CONCLUSION The polysaccharide contents of Dendrobii officinalis from differ- ent sources were diverse , all of which accorded with the standard of Chinese Pharmacopoeia： The content of polysaccharide in Dendrobium officinale from Yunnan province exceeded that from Zhejiang province, and the mannose content in biennial samples were higher than annual samples.