依达拉奉对体外循环大鼠脑损伤的保护作用

Protective effects of edaravone on cardiopulmonary bypass induced brain injury in rats

目的观察依达拉奉对体外循环（CPB）大鼠脑损伤的影响。方法成年雄性SD大鼠24只，体重400-450g，采用随机数字表法，均分为三组：假手术组（S组）、CPB组（C组）和依达拉奉组（E组）。S组穿刺但不CPB；C组建立CPB模型；E组建立CPB模型并于预冲液中加入依达拉奉5mg／kg。于术后24h经股动脉抽血检测血清白细胞介素-6（IL-6）、IL-10、神经元特异性烯醇化酶（NSE）和S-100β蛋白含量；取脑组织匀浆，测丙二醛（MDA）含量和超氧化歧化酶（SOD）活性。结果与S组比较，C组和E组IL-6、IL-10、NSE、S-100β和MDA含量明显升高（P〈0．05），SOD活性明显降低（P〈0．05）。与C组比较，E组IL-6、NSE、S-100β和MDA含量明显降低，IL-10含量、SOD活性明显升高（P〈0.05）。结论预冲液中加入依达拉奉5mg／kg可明显减轻CPB大鼠脑的炎症反应和氧化应激反应，对CPB大鼠有一定的脑保护作用。

Objective To observe the effects of edaravone on cardiopulmonary bypass （CPB） induced brain injury in rats. Methods Twenty-four Sprague-Dawley male rats, weighing 400-450 g, were equally randomized into 3 groups （n=8） ： sham operation group （group S）, CPB group （group C） and edaravone group （group E）. Rats in the group S were cannulated but did not undergo CPB. Rats in the groups C and E were subjected to CPB while the E group was also pretreated with edaravone 5 mg/kg in prime solutions. Blood samples were obtained via femoral artery 24 h after CPB to measure the serum concentrations of interleukin-6 （ IL-6 ）, interleukin-10 （ IL-10 ）, neuronespecificenolase （NSE） and S-10013 protein. The content of malondialdehyde （MDA） and the activity of superoxide dismutase （SOD） in brain tissue were also determined. Results Compared with the group S, the concentrations of IL-6, IL-10, MDA, NSE and S-100β protein increased while the activity of SOD decreased in the groups C and E （P〈0. 05）. Compared with the group C, the concentrations of IL-6, MDA, NSE and S-100O protein decreased while IL-10 and SOD increased in group E （P 〈 0.05 ）. Conclusion Edaravone 5 mg/kg pretreated in prime solutions can reduce inflammatory reaction and oxidative stress reaction, and exert protective effects on cardiopulmonary bypass induced brain injury in rats.