鸡传染性法氏囊病毒BJ-10株分离鉴定及其VP2基因序列分析

Isolation and Identification of IBDV BJ-10 Strain and Sequence Analysis of VP2 Gene

分离鉴定鸡传染性法氏囊病病毒(Infectious bursal disease virus,IBDV),并对其VP2基因进行序列分析。从陕西宝鸡地区疑似疫情的鸡群分离到1株IBDV(命名为BJ-10株),应用血清学琼脂凝胶免疫扩散试验(agar gel immunodiffusion test,AGID),结果在抗原孔和血清孔间可见明显的白色沉淀线。通过RT-PCR扩增VP2基因高变区并对其进行生物信息学分析,结果表明,BJ-10株与超强毒株中国香港HK46的核苷酸与氨基酸同源性分别为98.7%和99.6%,且高变区特征性氨基酸的变化与超强毒株完全一致,表明BJ-10株属于超强毒株,与香港地区的vvIBDV分离株有较为密切的亲缘关系。

The aim of this research is to isolate and identify infectious bursal disease virus （IBDV）, and analyze the sequence of VP2 gene. One strain of infectious bursal disease virus （named BJ 10 strain） was isolated from suspicious samples in Baoji city, Shaanxi province. Through serologic agar gel immunodiffusion test（AGID）, the results showed obvious white precipitation line between the antigen and serum hole. The highly variable region of VP2 gene was amplified by RT PCR from the strain and the sequence was used to do bioinformatic analysis. It was indicated that the homology of BJ 10 strain and the very virulent Hongkong HK46 strain reached 98.7% at nucleotides and 99.6% at amino acids, respectively. The characteristic amino acids in highly variable region were identical with that of other very virulent strains. The research results showed that BJ 10 strain belonged to vvIBDV, and closely related to the HK46 strain.