UPLC法测定元胡止痛胶囊中欧前胡素和异欧前胡素的含量

Determination of imperatorin and isoimperatorin in yuanhu zhitong jiaonang by UPLC

建立了以UPLC法测定元胡止痛胶囊中欧前胡素和异欧前胡素含量的方法。色谱柱为ACQUITY UPLC BEH C l8(1.7μm,2.1×50 mm),流动相为水-甲醇不同比例梯度洗脱,柱温30℃,流速0.3 mL/min,检测波长为248 nm。结果表明:欧前胡素在1.305～13.05μg/mL浓度范围内线性关系良好,回归方程为:Y=13828X+2962(r=0.9999),平均回收率为98.54%,RSD=0.76%,异欧前胡素在0.596～5.96μg/mL浓度范围内线性关系良好,回归方程为:Y=10634X+1128(r=0.9999),平均回收率分别为98.5%(RSD=0.76%)和98.9%(RSD=0.87%),欧前胡素的检出限为0.012 ng,定量限为0.04 ng,异欧前胡素的检出限为0.27 ng,定量限为0.92 ng。

To establish an UPLC method for detecting content of imperatorin and isoimperatorin in Yuanhu Zhitong Jiaonang. An ACQUITY UPLC BEH C l8（ 1. 7μm,2. 1 × 50 mm） was used,and the mobile phase was methanol and water by gradient elution mode,column temperature was 30 ℃,at a flow rate of 0. 3 ml / min and detection wavelength of 248 nm. The results showed that the linear range of imperatorin was 1. 305 ～13. 05μg / mL,the regression equation was as follows： Y = 13828X ＋ 2962（ r = 0. 9999）; The linear range of isoimperatorin was 0. 596 ～ 5. 96μg / mL,the regression equation was as follows： Y = 10634X ＋ 1128（ r =0. 9999）; The average recoveries were 98. 54%（ RSD = 0. 76%） and 98. 88%（ RSD = 0. 87%） respectively,the limit of detection of imperatorin and the limit of quantification of imperatorin were 0. 012 ng and 0. 04 ng,the limit of detection and the limit of quantification for isoimperatorin were 0. 27 ng and 0. 92 ng.