海带发酵产甲烷的初步研究

Preliminary study on methane production by fermentation of Saccharina japonica

通过大型海藻发酵生产甲烷,可以为清洁能源开发提供新的途径。试验以海带为原料,研究其发酵产气过程及甲烷的产量,并对发酵条件进行了优化。首先,利用单因素试验研究盐度、温度、起始pH对甲烷气体产量的影响;在此基础上,应用Box-Behnken中心组合设计建立数学模型,进行三因素三水平响应面分析。优化后的发酵条件:盐度6.58,温度28.92℃,起始pH 7.07。采用优化后发酵条件进行海带发酵,60 d总产气量达到6 310 ml,与优化前产气量(5 378 ml)相比提高了17.3%。

Methane produced by fermentation of macroalgae can provide a new route for the development of clean energy. The effective two-phase statistical method was used to optimize the fermentation conditions for the methane production by Sarccharina japonica. Firstly, the effects of fermentation condition parameters such as salinity, temperature and initial pH on methane fermentation were investigated by single-factor method. Subsequently, response surface methodology （RSM） based on a three-level, three-variable Box-Behnken experimental design was employed to evaluate interactive effects of three key factors including salinity, temperature and initial pH. The results of fermentation optimization were as follows： salinity 6.58, temperature 28.92 ℃, initial pH 7.07. And the total methane production of maeroalgae fermentation for 60 days was raised from 5 378 ml to 6 310 ml, a 17.3% increase compared to the initial fermentation conditions before the optimization.