外感风寒湿热对胶原诱导关节炎大鼠血清CD44水平的影响

Effect of Invasion of Wind Cold Damp and Heat Evil on CD44 in CIA Rats

目的:观察外感湿邪对胶原诱导关节炎(CIA)大鼠血清CD44水平的影响。方法:选取Wistar大鼠50只,随机分成5组,每组10只,分别为正常组、模型组、风寒湿组、风湿组及风湿热组,造模1个月后取大鼠踝关节滑膜进行病理切片,同时摘取眼球取血,用ELISA检测血清中CD44的变化情况。结果:模型组、风寒湿组、风湿组及风湿热组与正常组比较,滑膜细胞都有不同程度的增生,以风寒湿组增生最为明显,其次为风湿热组,风湿组次之,模型组增生程度最低;血清CD44水平均亦明显升高,差异有显著意义;5个实验组两两比较CD44值差异均有统计学意义。且各组血清CD44水平与其滑膜炎症程度呈正相关。结论:风、寒、湿、热等外邪对CIA发病是有影响的,影响程度以风寒湿组最为严重;在各造模组中,CD44水平明显升高,提示CD44可能参与了CIA的发生,且风、寒、湿、热等外在因素能加重CD44的表达,其中风寒湿组CD44表达值最高。

Objective： To observe the effect of the invasion of wind cold damp and heat evil on CD44 in collagen induced arthritis （ CIA ） rats. Methods ： In this research, the natural damp environment was simulated.50 Wistar rats were randomly divided into 5 groups： the normal group, model group, the wind cold damp （ WCD ） group, wind damp （ WD ） group and wind damp heat （ WDH ） group, each group only 10.The ankle synovial was taken out for pathological section at day 30 after experiment.The concentration of CD44 in serum was measured by enzyme-linked immunosorbent assay. Results： Compared with normal group, the levels of CD44 in model group, the wind cold damp group, wind damp group and wind damp heat group were obviously higher in the serum （ P〈0.01 ） .The increase in the wind cold damp group was the most obvious, followed by wind damp heat group, wind damp group was the second, and model group was the minimum （ P〈0.01 ） .And there was a positive correlation between the level of serum CD44 and synovial hyperplasia. Conclusion ： The invasion of wind cold damp and heat evil has an effect on the happening of CIA, and a^gravates the expression of CD44.