响应面法优化桑白皮甾醇微波辅助提取工艺

Optimization of Microwave-Assisted Extraction of Phytosterol from Mulberry Root Bark by Response Surface Methodology

为优化微波辅助提取桑白皮甾醇的工艺,分别考察微波功率、微波时间、液固比对桑白皮甾醇得率的影响;采用响应面法优化微波提取工艺,建立各因素与甾醇得率关系的回归方程。结果表明:最佳工艺条件为微波功率539W、微波时间41s、液固比35:1(mL/g),此时甾醇得率为(7.74±0.12)mg/g;实际值与理论值的相对误差为-4.21%,二者基本吻合。与溶剂法、超声波法相比,微波辅助法的甾醇得率分别提高了1.57%、19.07%,提取时间大大缩短。

This study was undertaken to optimize the microwave-assisted extraction of phytosterol from the root bark of mulberry （Morus alba L.） by response surface methodology. The yield of phytosterol was investigated with respect to three variables including microwave output power, irradiation time and solvent-to-solid ratio. As a result, a regression equation was fitted. The optimal conditions for phytosterol extraction were a solvent-to-solid ratio of 35：1 （mL/g） and microwave irradiation for 41 s with an output power of 539 W. The yield of phytosterol under the optimized conditions was （7.74 ±0.12） mg/g, showing a relative error of --4.21% compared with the predicted value and increases of 1.57% and 19.07% over those obtained by solvent extraction and ultrasonic-assisted extraction. Moreover, the extraction time was considerably shortened by using microwave-assisted extraction in comparison with the other two methods.