286株溶血葡萄球菌的临床分布及药敏分析

Clinical distribution and drug sensitivity analysis of 286 strains of Staphylococcus haemolyticus

目的了解宁波市妇女儿童医院溶血葡萄球菌的临床分布和耐药情况,为临床合理用药提供依据。方法对该院2010年1月1日至2012年12月31日期间分离的286株溶血葡萄球菌进行分析,采用法国生物梅里埃公司的VITEK-60型全自动微生物分析仪进行菌株鉴定和药敏试验,用参考菌株做质量控制。结果分离的溶血葡萄球菌主要来自儿科(59.8%);标本来源主要是阴道/尿道分泌物(29.7%),其次是创口分泌物(22.7%)。286株溶血葡萄球菌中耐甲氧西林溶血葡萄球菌(MRSH)261株,占91.3%;抗生素耐药分析显示,溶血葡萄球菌对青霉素G、苯唑西林、氨苄西林/舒巴坦表现出高度耐药性,耐药率分别为94.1%、91.3%、90.6%。对利福平、莫西沙星、呋喃妥因的耐药率较低,分别为2.8%、2.4%、1.0%;未发现耐万古霉素溶血葡萄球菌。结论本院溶血葡萄球菌对某些药物的耐药率很高,有必要对其进行耐药率监测,指导临床合理选择抗菌药物。

Objective To study the clinical distribution and drug resistance of S. haemolyticus isolated from Ningbo Women and Children＇s Hospital, and provide guidance for clinical medication. Methods 286 strains of S. haemolyticus isolated from the hospital during January 1, 2010 to December 31,2012 were collected. The clinical isolates were identified and their drug susceptivity were tested by using VITEK-60 automatic microbial analyzer of BioMerieux of France. And quality control was done with reference bacteria strains. Results Most of the isolates were from children （59.8%）. Most of the clinical specimens came from urethral/vaginal secretions （29.7%） and wound secretions （22.7%）. Of the 286 strains of S. haemolyticus, 261 strains were MRSH strains （91.3%）. The iso- lates were highly resistant to Penicillin G, Oxacillin and Ampicillin/sulbactam, and the resistance rates were 94. 1% , 91.3% and 90.6% respectively. The resistance rates of S. haemolyticus to Rifampicin, Moxifloxacin, Furan and Vancomycin were 2.8%, 2.4% , 1.0% and 0%. Conclusion The resistance rates of S. haemolyticus to several kinds of antibiotics were relatively high. It is important to strengthen the monitoring of drug resistance of S. haemolyticus to guide clinical use of antibiotics.