玉米秸秆低温纤维素分解菌的筛选及分解效果测定

Screening of Cellulose Degradation Bacteria Decomposing Maize Straw under Low Temperature and the Function Determining

在低温条件下,从腐烂的秸秆与土壤的混合样品中分离出降解纤维素能力强的4株菌株,测定这4株菌在玉米秸秆发酵中的CMC酶活。结果表明,菌株A14和A41在整个培养过程中CMC酶的活性比较高,分别为8.341 U/g和8.792 U/g。采用A14、A41处理玉米秸秆10 d,降解率分别达30.80%和31.52%。确定菌株A14和A41的最佳产酶条件为摇床转速150 r/min,接菌量为菌悬液(108个孢子/mL)与秸秆用量比1/10(质量比)。

Four strains of highly efficient cellulose degradation bacteria were screened from decomposing maize straw and soil under low temperature.Activities of carboxymethyl cellulose（CMC） of these 4 strains were assayed during maize straw fermentation.The results showed that the A14 strain and A41 strain had the higher activities of CMC,8.341 U/g and 8.792 U/g,respectively.After the treatments of A14 strain and A41 strain for 10 days,maize straw degradation rates were 30.80% and 31.52%,respectively.And the optimum conditions of A14 strain and A41 strain were as follows： rotation speed was 150 r/min,inoculated-pathogen quantities was decided by the inoculum of bacterial suspension（108spores/mL） and the dosage of the straw,which ensure that the proportion of the former and the latter was 1/10.