摘要
为探明砷胁迫对烟草生理特性的影响,选用本生烟草(Nicotiana benthamiana)为材料,采用盆栽试验,挑选了生长4周后健壮的烟草苗进行砷(As2O3)胁迫处理(50μg·L-1),并于处理后0、24、48、72和96h分别取样并进行相关生理指标(叶绿素含量、丙二醛含量、超氧化物歧化酶、过氧化物酶和过氧化氢酶活性)的测定.结果显示:随着砷处理时间的延长,本生烟草叶片叶绿素含量、超氧化物歧化酶和过氧化物酶活性呈先升后降趋势,过氧化氢酶活性呈下降趋势,丙二醛含量呈上升趋势.试验数据表明:短时间的砷胁迫烟草会出现自我保护现象,但胁迫超出一定时间(24h)后对烟草造成一定的伤害.
In order to elucidate the influence of arsenic on the related physiological indexes in tobacco, 4-week- old tobac- co Nicotiana benthamiana were treated with the arsenic concentration of 50 μg/L. The changes in the contents of chlorophyll and malondiaidehyde （MDA） , the activities o5 superoxide dismutase （SOl）） , peroxidase （POD） and catalase （CAT） were de- termined at 0 th, 24 th, 48 th, 72 nd and 96 th hours after the treatment. The results showed that the content of chlorophyll, the activities of SOD and POD were first increased and then decreased, the activity of CAT was decreased, and the content of MDA was increased. These data suggested that the endurance to arsenic stress was utmost in tobacco Nicotiana henthamiana , and they would be injured beyond this limit.
出处
《河南师范大学学报（自然科学版）》
CAS
北大核心
2013年第5期144-147,共4页
Journal of Henan Normal University(Natural Science Edition)
基金
国家自然科学基金(31260344)
内蒙古自然科学基金(2011BS0506,2012MS0301)
内蒙古自治区高等学校科学研究项目(NJZZ11139)
内蒙古自治区教育厅“青年科技英才支持计划”
关键词
烟草
砷胁迫
生理特性
Nicotiana benthamiana
arsenic stress
physiological indexes
