常用消毒剂对部分多重耐药菌杀灭效果的观察

OBSERVATION ON THE BACTERICIDAL EFFECT OF USUALY USED DISINFECTANT TO PART MULTIDRUG-RESISTANT BACTERIA

目的观察常用消毒剂对临床分离多重耐药菌杀灭效果。方法采用悬液定量杀菌试验方法,对医院常用的含氯消毒剂、碘伏、戊二醛和含乙醇快速手消毒剂对多重耐药MRSA和肠球菌杀灭效果进行了实验室观察。结果临床分离的金黄色葡萄球菌、屎肠球菌、粪肠球菌对常用抗生素存在不同程度的耐药。用含有效氯250 mg/L的含氯消毒剂、125 mg/L戊二醛消毒液、有效碘50 mg/L的碘伏消毒剂、16倍稀释的某含乙醇氯己定快速手消毒剂对临床分离的MRSA和金黄色葡萄球菌标准菌株作用5 min,平均杀灭率均达到90%以上,作用10 min可达到完全杀灭。结论上述临床分离的部分多重耐药菌对医院常用化学消毒剂耐受性与标准菌株一致。

Objective To observe the bactericidal effect of usualy used disinfectant to part muhidrug - resistant bacteria. Methods Suspension quantitative bactericidal test was performed to investigate bactericidal effect of chlorine - containing disinfectant, glutaraldehyde, iodophor and alcohol - based hand - disinfectant on multidrug - resistant MRSA and enterococcus. Results Clinical isolates of Staphylococcus aureus, Enterococcusfaecalis and Enterococcus feces have different degrees of resistance to commonly used antibiotics. When we used 250 mg/L chlorine - containing disinfectant, 125 mg/L glutaraldehyde disinfectant solution, 50mg/L available iodine, 16 times diluted alcohol -based hand -disinfectant to clinical isolated of MRSA and standard Staphylococcus aureus for 5 min, the average killing rate can reach more than 90%, 10min can achieve completely killed. Conclusion The survivability of part.clinical isolated muhidrug - resistant bacteria was in accord with standard strain to usual used chemical disinfectants.