一种胍盐中和剂悬液定量鉴定试验方法研究

STUDY ON METHOD OF EFFICACY VERIFICATION OF NEUTRALIZER FOR SUSPENSION QUANTITATIVE GERMICIDAL TEST

目的研究中和剂悬液定量鉴定试验的补充方法。方法采用TSA倾注法定量培养与营养肉汤定性培养法相结合的方法,鉴定聚六亚甲基胍盐酸盐中和剂的中和效果。结果选用0.0025 mol/L聚乙烯基硫酸钾(PVSK)作为2.0 g/L聚六亚甲基单胍盐的中和剂,TSA倾注法第2组无菌生长,而采用营养肉汤定性培养法第2组有菌生长。营养肉汤培养更适合受损细菌的恢复生长。结论在使用TSA倾注法进行中和剂悬液定量鉴定试验时,如第2组出现无菌生长,可同时对第2组进行营养肉汤定性培养,佐证中和剂的中和效果。

Objective To study a compensative method of efficacy verification of neutralizer for suspension quantitative germicidal test. Methods The quantitative cultivation by TSA pouting and qualitative cultivation by nutrient broth were used to evaluate the effectiveness of neutralizer for neutralizing polyhexamethylene guanidine hydrochloride （PHMG - HCl）. Results A standard solution of potassium poly（vinyl sulfate） （PVSK） was used as the neutralizer of a disinfection solution containing 2.0 g/L PHMG - HC1 in the test. The second group had no bacterial growth which cultured by TSA pouring, while the bacterial in the second group grew normally cultured by nutrient broth. The germieuhure method of nutrient broth was more suitable for impaired bacterial recovery growth. Conclusion In neutializer identify test,when the second group show no bacterial growth by TSA pouring, we could identify the second group by neutrient broth to prove the conclusion.