期刊文献+

高肺血流性肺动脉高压大鼠肺动脉平滑肌细胞钙激活氯离子通道的电生理研究 被引量:1

Study of electrophysiology of Ca2 +-activated Cl-channels in pulmonary arterial smooth muscle cells of pulmonary hypertension induced by high pulmonary blood flow in rats
原文传递
导出
摘要 目的 观察肺动脉平滑肌细胞(PASMCs)钙激活氯离子通道(ClCa)的电生理特性,探讨其在高肺血流性肺动脉高压(PAH)形成过程中的可能作用.方法 SD大鼠40只,随机分为3组:对照组(10只)、假手术组(10只)、模型组(20只).建立模型成功后同条件下饲养11周,用右心导管法测定大鼠右心室压力(RVSP),并检测右心室肥厚指数(RVHI);急性酶分离法分离出单个PASMC,应用膜片钳全细胞记录模式,测定各组大鼠PASMCs静息膜电位(Em)、ClCa电流(IClCa)及电流密度的改变,比较各组大鼠Ⅰ-Ⅴ曲线的变化,并进行相关性分析.结果 与对照组和假手术组比较,模型组大鼠ClCa内向的尾电流(Itail)的Ⅰ-Ⅴ曲线明显向下移;对照组与假手术组间差异无统计学意义.静息Em与RVSP、RVHI呈正相关(P均<0.01);Itail的电流密度与RVSP、RVHI、静息Em均呈负相关(P均<0.01).结论 腹主动脉-下腔静脉分流所致高肺血流性PAH大鼠,随着肺动脉压的增高,PASMCs的静息Em升高,ClCa的Itail的绝对值增大,Ⅰ-Ⅴ曲线下移,提示ClCa电流的改变可能在高肺血流性PAH的形成过程中起一定作用. Objective To investigate the characteristics of different electrophysiology of Ca2+-activated Cl-channels (ClCa) in pulmonary arterial smooth muscle cells (PASMCs) between pulmonary arterial hypertension (PAH) rats induced by left-to-right shunt and normotensive rats,and to study its possible role in the progress of PAH induced by high pulmanry blood flow.Methods Forty SD rats were randomly divided into 3 groups:control group (n =10),sham group (n =10),PAH model group (n =20).After molding,the rats were raised in the same condition for 11 weeks.Right ventricular systolic pressure (RVSP) of each rat was measured by right cardiac catheterization procedure.Right ventricular hypertrophy index (RVHI) was calculated.Single PASMC was obtained by acute enzyme separation method and the conventional whole-cell patch clamp technique was used to record the resting membrane potential (Em),ClCa and current density.The Ⅰ-Ⅴ curve between each group were compared.Results Compared with control group and sham group,the Ⅰ-Ⅴ curve about Itail of PAH model group was significantly shift downward; the difference between control group and sham group was not significant.There were positive correlations between Em and RVSP,RVHI (all P < 0.01),and negative correlations between Itail and RVSP,RVHI and Em(all P < 0.01).Conclusions During the formation process of left-to-right shunt induced PAH,with the step up of pulmonary arterial pressure,the Em of PASMCs stepping up.The absolute value of current density of inward ClCa currents was increased,and its Ⅰ-Ⅴ curve was shift downward.These suggest that the change of ClCa currents may play a role in the PAH induced by left-to-right shunting.
出处 《中华实用儿科临床杂志》 CAS CSCD 北大核心 2013年第18期1422-1426,共5页 Chinese Journal of Applied Clinical Pediatrics
基金 国家自然科学基金(81160040) 广西自然科学基金(桂科自0991133)
关键词 左向右分流 肺动脉高压 钙激活氯离子通道 肺动脉平滑肌细胞 膜片钳 Left-to-right shunt Pulmonary arterial hypertension Ca2 +-activated Cl-channel Pulmonary artery smooth muscle cell Patch-clamp technique
  • 相关文献

参考文献15

  • 1王涛,蔡雯雯,刘翰旻.波生坦治疗儿童肺动脉高压的研究进展[J].实用儿科临床杂志,2010,25(2):147-150. 被引量:6
  • 2杜军保;唐朝枢.肺动脉高压[M]北京:北京大学医学出版社,2010193-212.
  • 3Mulder BJ. Changing demographics of pulmonary arterial hypertension in congenital heart disease[J].European Respiratory Review,2010,(118):308-313.
  • 4Berg J,Yang H,Jan LY. Ca2 +-activated Cl-channels at a glance[J].Journal of Cell Science,2012,(Pt 6):1367-1371.
  • 5高扬,齐建光,李晓惠,庞璐璐,金红芳,杜军保.肾上腺髓质素对大鼠高肺血流性肺血管结构重构的干预作用[J].实用儿科临床杂志,2012,27(22):1751-1753. 被引量:10
  • 6梁晨,金红芳,杜淑旭,杜军保.野百合碱诱导肺动脉高压大鼠肺动脉Ⅰ型胶原的变化[J].实用儿科临床杂志,2010,25(1):18-20. 被引量:11
  • 7Angermann JE,Forrest AS,Greenwood IA. Activation of Ca2 +-activated Cl-channels by store-operated Ca2 + entry in arterial smooth muscle cells does not require reverse-mode Na +/Ca2 + exchange[J].Can J Physiol Phannacol,2012,(07):903-921.
  • 8Sun H,Xia Y,Paudel O. Chronic hypoxia-induced upregulation of Ca2+-activated Cl-channel in pulmonary arterial myocytes:a mechanism contributing to enhanced vasoreactivity[J].Journal of Physiology,2012,(Pt 15):3507-3521.
  • 9Wang J,Haanes KA,Novak I. Purinergic regulation of CFTR and Ca2 +-activated Cl-channels and K + channels in human pancreatic duct epithelium[J].American Journal of Physiology-Cell Physiology,2013,(07):C673-C684.
  • 10Forrest AS,Joyce TC,Huebner ML. Increased TMEM16A-encoded calcium-activated chloride channel activity is associated with pulmonary hypertension[J].American Journal of Physiology-Cell Physiology,2012,(12):C1229-C1243.

二级参考文献88

  • 1刘确,关永源,曾进胜,贺华,孙家钧.硝苯地平和环匹阿尼酸对易卒中型肾性高血压大鼠血管平滑肌收缩功能的影响[J].中国药理学与毒理学杂志,1996,10(1):34-38. 被引量:3
  • 2齐建光,丁亚光,唐朝枢,杜军保.肾上腺髓质素_(1-50)对大鼠慢性低氧性肺动脉高压的调节作用[J].北京大学学报(医学版),2006,38(2):151-154. 被引量:5
  • 3石琳,杜军保,卜定方,齐建光,唐朝枢.NO前体对高肺血流时肺动脉胱硫醚-γ-裂解酶基因表达的调节作用[J].中国应用生理学杂志,2006,22(3):343-347. 被引量:1
  • 4黄如训 曾进胜.肾性高血压大鼠脑血管病变的初步观察[J].中国神经精神疾病杂志,1990,16:136-138.
  • 5Galie N, Manes A, Palazzini M, et al. Management of pulmonary arterial hypertension associated with congenital systemic - to - pulmonary shunts and Eisenmenger's syndrome[ J]. Drugs ,2008,68 ( 8 ) : 1049 - 1066.
  • 6Beghetti M, Hoeper M, Kiely DG, et al. Safety experience with bosentan in 146 children 2 - 11 years with pulmonary arterial hypertension: Resuits from the European post - marketing surveillance programme [ J ]. Pediatr Res, 2008,64 ( 2 ) :200 - 204.
  • 7Tulloh R. Etiology, diagnosis, and pharmacologic treatment of pediatric pulmonary hypertension [ J]. Pediatr Drugs ,2009,11 (2) : 115 - 128.
  • 8D'Alonzo G,Barst R,Ayres S,et al. Survival in patients with primary pulmonary hypertension: Results from a national prospective registry [ J ]. Ann Intern Med, 1991,115 (5) :343 - 349.
  • 9Haworth SG, Hislop AA. Treatment and survival in children with pulmonary arterial hypertension:The UK pulmonary hypertension service for children 2001 - 2006 [ J ]. Heart,2009,95 (4) :312 - 317.
  • 10De Wolf D. Clinical practice : Pulmonary hypertension in children [ J ]. Eur J Pediatr,2009, 168 (5) : 515 - 522.

共引文献29

同被引文献8

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部