提高白菜离体培养植株再生频率的研究

Enhancement of Plant Regeneration Frequency of in Vitro Cultured Chinese Cabbage

选用 7个白菜类蔬菜栽培品种和 1个杂交亲本进行离体培养植株再生试验 ,筛选出最佳培养基配方为 1/2倍NH+4 浓度的MS培养基附加BAP 2mg/L、NAA 0 .4 5mg/L和AgNO37.5mg/L ,大幅度提高了植株的再生频率 ,高达 84 .8% ,大大缩短成苗周期 ;子叶—子叶柄接种后 ,最早的仅需 6d即可分化出不定芽 ,从接种到再生苗的移栽最快的仅需 4 0d ,平均一批约需 50d ,达到了高频快速的要求 ;该培养基对白菜类蔬菜的不同品种有着较广的适应性。对不定芽的发生过程进行组织学观察表明 ,在本试验条件下 ,白菜离体培养植株再生方式为外植体直接出芽。再生苗生根后移栽的成活率可达 95%以上。

An efficient and fast method for plant regeneration from petiole explants with cotyledon was developed in Chinese cabbage( Brassica campestris ssp. chinensis Makino).A MS medium with half strength of NH + 4 concentration and supplemented with BAP 2mg/L,NAA 0.45mg/L and AgNO 3 7.5mg/L was found to be optimum for Chinese cabbage in obtaining the high frequency of shoot regeneration.The frequency of shoot regeneration of 7 Chinese cabbage cultivators and 1 parentage reached as high as 84.8%.The little shoots could be observed 6 days after inoculation and shoots were formed directly.The survival rate of planets after transplanting to soil reached as high as 95%.