砂地柏叶中鬼臼毒素的分离与鉴定

The isolation and identification of podophyllotoxin from Sabina vulgaris Ant.

在生物活性跟踪测试指导下 ,从砂地柏叶的乙醇提取物中通过硅胶柱层析 ,分离得到一种对菜青虫有高拒食活性的成分 ,经紫外光谱 (UV)、红外光谱 (IR)、质谱 (MS)、氢核磁共振谱 (1H- NMR)分析 ,鉴定其为鬼臼毒素。其对菜青虫在高质量浓度下呈拒食作用 ,低质量浓度下则具胃毒毒杀作用。

According to the guidance of bioactivity assaying and using silica column chromatography,a compound having high antifeeding activity to the tested insect Pieris rapae L.is isolated from the ethanol extract of the leaves of Sabina vulgaris Ant..Its molecular structure is identified to be podophyllotoxin,a known compound,by UV,IR,MS and 1H-NMR.It is also found that the podophyllotoxin has antifeeding activity in higher concentrations and has stomach poisionous activity in lower concentrations to the 5 instar larva of Pieris rapae L..