环境因素对小麦苗期SOD、MDA及可溶性蛋白的影响

Effect of different environmental factors on SOD activity, MDA and soluble protein contents of wheat leaves

采用盆栽试验 ,模拟干旱胁迫、铜污染 (三级农田 )及不同施肥状况 ,测定了小麦苗期的生物量及叶片中的 SOD活性、MDA和可溶性蛋白含量。结果表明 ,水分、铜污染和施肥三因素对小麦苗期的生物量都有显著影响 ,表现为水分 >施肥 >铜污染 ;水分对 MDA含量的影响大于铜污染和施肥 ,表现为干旱可显著增大叶片 MDA含量 ,并使 MDA/SOD值增大 ;对 SOD和可溶性蛋白 ,水分和铜污染的影响大于施肥的影响 ,表现为干旱胁迫下 ,SOD活性升高 ,可溶性蛋白含量降低 ,而铜污染处理使可溶性蛋白含量升高 ;

The growth and SOD activity,MDA and soluble protein contents of wheat leaves at seedling stage were determined in simulating soil basin culture,with controlled copper content (Cu: 400 mg/kg in dry soil and background soil),irrigated water (at 70% and 35% of soil capillary water) and fertilization (CK,N and NP).The results indicated that:(1) The level of water,fertilization and copper had notable effects on wheat growth,and the effect degree was water>fertilization>copper.(2) Water had greater effect on MDA content than fertilization and copper,and drought stress obviously increased the MDA content and MDA/SOD values.(3) Water and copper had greater effect on SOD activity and soluble protein content.Drought stress increased SOD activity,decreased soluble protein content,and copper stress increased soluble protein content.(4) There existed interaction on wheat growth among the three different environmental factors.