晋南种公牛染色体的监测

Chromosomal detection in Jinnan stud bull

通过淋巴细胞培养法对拟选入精子库的 1 3头晋南种公牛的染色体进行了研究和监测 ,结果表明 ,晋南牛 Y染色体存在多态性 ,具有中 (亚中 )部 (7头 )和近端 (6头 )着丝粒 Y染色体双重核型 ;对其体细胞染色体畸变类型和频率分析表明 ,染色体多倍体有三倍体、四倍体和六倍体 ,其总畸变率为 4.1 3%。结构畸变的类型有染色单体缺失、断裂、间隙和染色体间隙、着丝点断裂等 ,其总畸变率为 2 .54%。经比较分析 ,研究的 1 3头晋南种公牛染色体的畸变率在正常范围内。

By using the lymphocyte culture,the study and detection on chromosomes in 13 selected Jinnan bulls which will be selected into bovine spern pool are done.The result shows that Jinnan cattles Y chromosomes have polymorphism possessing dual-karyotype phenomenon of 6 acrocentric and 7 metacentric (submetacenrtic) Y chromosomes.The results on the types and frequencies of chromosomal aberration of somatic cells in Jinnan cattle show that chromosomal polyploid include:triploid,tetraploid and hexaploid with the occurrence rate of 4.13%,that the types of structural aberration include:chromatin delletion,breakage and gap,chromosome gap and centromere breakage et al.with a structural aberration rate of \{2.54%\}.Through comparative analysis,the chromosomal aberration rate of 13 Jinnan stud bulls is in normal range in this study.