mRNA差异显示技术及其在植物生理研究中的应用

The theory of DDRT-PCR and its applications in plants

简述了 m RNA差异显示技术的基本原理及其在基因表达差异、基因的鉴定与克隆、激素调控机理、抗逆性机理等方面的应用 。

mRNA differential display reverse transcription-PCR(DDRT-PCR) is a useful technique for analyzing differential gene expression at transcription level.The theory and characters of mRNA differential display (DDRT-PCR) and its applications to differentially expressed gene,gene cloning,the mechanism of hormone regulation and control,and resistance to adverse are reviewed.The main problems of mRNA differential display and its improved methods are introduced in the paper.