3,4-双(4'-氨基呋咱基-3')氧化呋咱对兔眼刺激性试验研究

Experimental study on eye irritation of DATF in rabbits

目的评价3,4-双(4'-氨基呋咱基-3')氧化呋咱(DATF)对大耳白兔眼睛是否有刺激作用及其程度。方法选择4只大耳白兔,采用自身对照方式,分别用DATF 100 mg对大耳白兔左侧眼睛进行给药,于给药后1、24、48、72 h和4、7 d采用裂隙灯观察大耳白兔的双眼角膜、虹膜和结膜,对其损伤程度参照评分标准进行评分。结果 DATF对兔眼角膜、虹膜、结膜均有刺激性,并伴有充血、水肿和分泌物,给药后1和24 h眼刺激总积分均值分别为43和94,48 h后各观察点总积分均值都达到最高评分值110,对照右眼的总积分均值都为0。结论 DATF对大耳白兔眼睛具有重度刺激性。

[ Objective ] To evaluate whether the 3,4-bis { 4＇-aminofurazano-3＇ } furoxan { DATF ） has irritation on rabbit＇ s eyes and the extent of reaction. [ Methods] The left eye of 4 rabbits were exposed to the dose of 100 rag. The self-contrast test of rabbit eyes were observed in study. The cornea, iris and conjunctiva of rabbit eyes were observed with slit lamp 1 hour, 24 hour, 48 hour, 72 hour, 4 day and 7 clay after the DATF administration. The level of eye injury was evaluated according to the standard. [ Results] DATF produced irritation in the rabbit eyes of cornea, iris and eonjunctiva, which associated with congestion, edema and secre- tions. The total score of eye irritation at 1 hours and 24 hours were 43 and 94 respectively. And it reached the highest of 110 after 48 hours. All of the comparison value was 0. [ Conclusion] DATF has severe irritation effects on the eyes of rabbit.