模拟晕船刺激对大鼠糖代谢及血清中铬和锶水平的影响

Effect of motion sickness stimulus on glycometabolism and serum chromium,strontium concentrations

目的探讨短时模拟晕船刺激后大鼠血糖变化规律及血清中铬、锶的变化情况,为晕动病发生机制的全面理解以及长期晕刺激条件下矿物元素膳食与补充研究提供初步实验依据。方法健康雄性SD大鼠随机分为空白对照组,晕船刺激组。晕船刺激组置于晕船旋转装置中旋转30 min。晕刺激后立即测血糖,并观察记录晕反应指数,作为晕动病发生情况的行为学评价指标。空白对照组与晕船刺激组一同处死取血,电感耦合等离子体(ICP)检测血清中元素铬锶含量。放免法(RIA)测血清中胰岛素水平。结果晕船刺激引起晕反应指数增加,血清中铬水平明显下降,胰岛素水平下降,血糖上升,与对照组比较,差异均有统计学意义(P<0.05)。模拟晕船刺激30 min血清中锶变化水平与对照组的差异无统计学意义。结论短时晕刺激影响糖代谢水平,且引起糖代谢相关元素铬发生变化。短时刺激情况下血清铬降低幅度尚在正常水平,长期刺激是否会引起血清铬病理性降低及补充铬是否会减缓晕动病症状甚至降低晕动病的发生值得深入探讨。

[Objective]To study the glycemic variation and the changes of serum chromium（ Cr} , strontium（ Sr} after short-term motion sickness stimulus, and provide initial experimental evidences for comprehensive understanding of the mechanisms of motion sickness, and study of minerals and dietary supplement under long-term motion stimulus. [ Methods ] Healthy male SD rats were randomly divided into seasickness stimulus group and control group. Seasickness stimulus group rats were stimulated in a rotation device for 30 minutes. Motion sickness index for each rat was observed and recorded at the end of seasickness stimulus, as an eval- uation method for motion sickness. After measuring the blood glucose level, the rats in the 2 groups were decapitated immediately with blood at same time. Serum Cr and Sr were determined by ICP. And serum insulin was determined by RIA. [ Results] Seasick stimulus induced motion sickness index increased （ P 〈 0.05 ） , serum Cr concentration decreased, serum insulin levels decreased, and had a significantly higher blood glucose level than that in control group （ P 〈 0.05 ）. Serum Sr concentration didn ＇ t change sig- nificantly after 30-minute seasickness stimulation. [ Conclusion] Short-term motion sickness stimulus can affect blood glucose level and induce serum Cr concentration change. The serum Cr concentration is still in normal range after short-term motion sickness stimulus. It is worthy to investigate whether long-term motion sickness stimulation can induce pathological reduction of serum Cr and whether chromium supplementation can relieve symptoms of motion sickness, even decrease its incidence.