丝素蛋白表面微观形貌对血管内皮细胞增殖功能的影响

Influence of Microtopographic Structures of Silk Fibroin on the Proliferation of Vascular Endothelial Cell

目的:研究4种不同微观形貌的丝素蛋白膜对血管内皮细胞生长和增殖的影响,以寻找理想的丝素蛋白表面形貌,为生物材料应用于血管腔内治疗提供新的思路。方法:采用软印刷技术制备各种微观形貌的丝素蛋白膜,镜下观察丝素蛋白膜的不同微观形貌对血管内皮细胞形态以及行为的影响,采用磺基罗丹明B(sulforhodamine B,SRB)法观察丝素蛋白表面微观形貌对血管内皮细胞活性的影响。结果:内皮细胞在丝素蛋白材料上可以贴附生长,不同方式的表面微观形貌雕刻对于血管内皮细胞生长活性的影响不同;形貌2、3、4效果明显好于对照组(P<0.05),形貌2雕刻的细胞生长优势最为明显;形貌5雕刻效果与对照组比较,差异无统计学意义(P>0.05)。结论:丝素蛋白覆膜材料能支持血管内皮细胞的正常生长和增殖,并可以通过丝素蛋白膜表面的微观形貌影响血管内皮细胞的形态及生物学行为。

Objective：To search the ideal surface morphology of the silk fibroin by studying four different microtopographic structures of silk fibroin and their effects on the proliferation of vascular endothelial cells,in order to provide a new idea for applying biological materials in endovascular treatment.Methods：The soft printing technology was used to prepare a variety of morphologies of silk fibroin.The effects of microtopographic structures of silk fibroin on the morphology of vascular endothelial cells and celluar behavior were observed under microscope,and sulforhodamine B（SRB） test was used to detect cell activity.Results： Vascular endothelial cells could attach and grow on different silk fibroins.Microtopographic structures of silk fibroin might lead to different growth behaviors of vascular endothelial cells.The effects of microtopographic structure 2,3,4 were significantly better than that of structure 1（the control group） P0.05,and cell growth advantage in structure 2 was the most obvious.The effect of structure 5 had no significant difference compared with that of the control group（P0.05）.Conclusions： The microtopographic structure of silk fibroin has positive effects on vascular endothelial cell morphology,attachment and proliferation,indicating that the microtopographic structure can affect the biological behavior of endothelial cells.