禁食时间对链脲佐菌素诱导1型糖尿病小鼠模型的影响

Effect of Different Fasting Time on the Construction of Type 1 Diabetic Mice Model Induced by Streptozotocin

目的:研究不同禁食时间对多次小剂量注射链脲佐菌素(streptozotocin,STZ)诱导1型糖尿病模型的影响,探讨其最佳禁食时间。方法:将40只C57BL/6J小鼠随机分为4组:对照组(C组)、禁食4 h组(F4组)、禁食10 h组(F10组)和禁食16 h组(F16组),每组10只。禁食组小鼠按多次小剂量腹腔注射STZ的方法诱导1型糖尿病模型,C组注射等量的柠檬酸盐缓冲液。测定各组小鼠注射STZ前及注射后1、2、3、4周的血糖、体质量的变化,计算小鼠糖尿病模型的成功率。结果:注射STZ2周后,各禁食组小鼠体质量明显降低,与C组比较差异有统计学意义(P<0.05或P<0.01)。注射STZ2周时,各禁食组血糖水平均>11.1 mmol/L,与C组比较差异有统计学意义(P<0.01);随着禁食时间的延长,血糖水平逐渐升高,自第3周起,F10组与F4组的血糖水平差异有统计学意义(P<0.05),但与F16组比较差异无统计学意义(P>0.05)。此外,禁食10 h后注射STZ,模型成功率为90.0%,同时不引起明显的体质量下降。结论:采用多次小剂量注射STZ的方法诱导小鼠1型糖尿病模型时,每次注射STZ前最佳禁食时间为10 h。

Objective：To investigate the effect of different fasting time on the construction of type 1 diabetic mice model induced by multiple low-dose streptozotocin（STZ）,in order to determine the appropriate fasting time.Methods：A total of 40 C57BL/6J mice were randomly divided into four groups：control group（Group C）,fasting for 4 hours group（Group F4）,fasting for 10 hours group（Group F10）and fasting for 16 hours group（Group F16）.Mice in fasting groups were injected with STZ,while mice in control group were injected with equal volume of citrate buffer.The non-fasting blood glucose and body weight before injection,as well as 1,2,3,4 weeks after injection were detected.Then the diabetic incidences were calculated.Results：The non-fasting blood glucose level and diabetic incidence in Group F10 were higher than those in Group F4,but there was no significant difference between Group F10 and Group F16.In addition,the diabetic incidence was 90% by STZ injection after fasting for 10 hours,but the body weight of mice had no significant decrease.Conclusions：The optimum fasting time is 10 hours before STZ injection in the construction of type 1 diabetic mice model induced by multiple low-dose STZ.