宣肺止嗽汤对感染后咳嗽大鼠肺泡灌洗液中细胞因子的影响

Effect of Xuanfei Zhisou Decoction(宣肺止嗽汤)on Bronchoalveolar Lavage Fluid Cell Factor in Rats with Post Infectious Cough

目的观察宣肺止嗽汤对感染后咳嗽大鼠肺泡灌洗液(BALF)中白介素-4(IL-4)、白介素-5(IL-5)、白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)的影响。方法以烟熏加脂多糖滴鼻加辣椒素刺激法制备大鼠感染后咳嗽模型,造模成功后随机分为模型组、宣肺止嗽汤低、中、高剂量组和惠菲宁阳性对照组,另取健康雄性SPF大鼠10只设为正常组。实验第21天开始给药干预,治疗2周后采用脱椎法处死大鼠,采用ELISA检测各组大鼠BALF中IL-4、IL-5、IL-8及TNF-α水平。结果模型组与正常组比较,大鼠BALF中IL-4、IL-5、IL-8、TNF-α水平显著升高,而治疗组与模型组比较,大鼠BALF中IL-4、IL-5、IL-8、TNF-α水平显著降低,与正常组接近。结论宣肺止嗽汤可能通过抑制炎症细胞释放细胞因子,减轻气道炎症,降低气道高反应,从而有效的治疗感染后咳嗽。

Objective To observe the effect of Xuanfei-Zhisou Recipe on post infectious cough rats interleukin solution 4, interleukin 5, interleukin 8, tumor necrosis factor-α, in BALE. Methods Smoke plus lip polysaccharide nasal combined with capsaicin preparation of rat post infection cough model （proven）, the rats were randomly divided into model group,Xuanfei- Zhisou Recipe low dose, Xuanfei-Zhisou Recipe middle dose, Xuanfei-Zhisou Recipe high dose group, positive control group and Whifinin, another 10 healthy male SPF rats as normal group. The twenty-first day of the experiment to drug intervention, 2 weeks after the treatment, the rats were sacrificed and vertebral method, using ELISA to detect BALF rats of group IL-4, IL-5,IL-8 and TNF-α levels. Results Comparison between the model group and the normal group, IL-4, IL-5, IL-8, TNF- αlevel in rats with BALF increased significantly, while the treatment group compared with the model group, IL-4, IL-5, IL-8, TNF-α level in rats with BALF significantly reduced amd is close to the normal group. Conclusion Xuanfei-Zhisou decoc- tion could inhibit the release of cytokines, inflammatory cells, alleviate the airway inflammation, reduce airway hyperrespon- siveness, and effective in the treatment of cough after infection.