UPLC测定竹叶青酒中橙皮苷成分的研究

Study on Determination of Hesperidin in Zhuyeqing Liquor by UPLC

目的：采用超高效液相色谱法（UPLC）建立保健酒竹叶青中橙皮苷的含量测定方法。方法：采用ACQUITYUPLCBEHC18色谱柱（1．7μm，2．1×50mm），流动相为混合流动相（甲醇：醋酸：水=35：4：61）：水=72：28，检测波长283nm，柱温30℃。结果橙皮苷进样量在0．0021-0．126μg范围内，与峰面积线性关系良好，橙皮苷的平均回收率为95％，相对标准偏差（RSD）为1．8％（n=3）。结论：该方法简便、快速，准确、重复性好，可用于竹叶青酒中橙皮苷的检测。

OBJECTIVE To establish an UPLC method for the determination of hesperidin in Zhuyeqing Liquor. METHODS BEH C18 column （1.7m,2.1 mm × 50mm）was used, and The mobile phase was mixed mobile phase （methanol： acetic acid： water = 35 ： 4 ： 61） ： water = 72：28.Detection wavelength was at 283nm at 30℃. RESULTS Within the range of 0.0021 to 0.126 μg,hesperidin had a good linear relation to the peak area. The average recovery rate of Hesperidin was 95%, and RSD was 1.8%（n=3）. Conclusion The method was accurate and the reproducibility was good. It is suitable to analyze hesperidin in Zhuyeqing Liquor using by UPLC method.